Nucleotide analogues as probes for DNA polymerases

Jung, Karl-Heinz; Marx, Andreas

Nucleotide analogues as probes for DNA polymerases

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Date

2005

Publication type

Journal article

Publication status

Published

Published in

Cellular and Molecular Life Sciences : CMLS ; 62 (2005), 18. - pp. 2080-2091. - ISSN 1420-682X. - eISSN 1420-9071

Abstract

Transmission of the genetic information from the parental DNA strand to the offspring is crucial for the survival of any living species. In nature, all DNA synthesis in DNA replication, recombination and repair is catalyzed by DNA polymerases and depends on their ability to select the canonical nucleobase pair from a pool of structurally similar building blocks. Recently, a wealth of valuable new insights into DNA polymerase mechanisms have been gained through application of carefully designed synthetic nucleotides and oligonucleotides in functional enzyme studies. The applied analogues exhibit features that differ in certain aspects from their natural counterparts and, thus, allow investigation of the involvement and efficacy of a chosen particular aspect on the entire complex enzyme mechanism. This review will focus on a depiction of the efforts that have been undertaken towards the development of nucleotide analogues with carefully altered properties. The different approaches will be discussed in the context of the motivation and the problem under investigation.

Subject (DDC)

540 Chemistry

Cite This

ISO 690

JUNG, Karl-Heinz, Andreas MARX, 2005. Nucleotide analogues as probes for DNA polymerases. In: Cellular and Molecular Life Sciences : CMLS. 62(18), pp. 2080-2091. ISSN 1420-682X. eISSN 1420-9071. Available under: doi: 10.1007/s00018-005-5117-0

BibTex

@article{Jung2005-09Nucle-33963,
  year={2005},
  doi={10.1007/s00018-005-5117-0},
  title={Nucleotide analogues as probes for DNA polymerases},
  number={18},
  volume={62},
  issn={1420-682X},
  journal={Cellular and Molecular Life Sciences : CMLS},
  pages={2080--2091},
  author={Jung, Karl-Heinz and Marx, Andreas}
}

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