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Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes

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1997

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Rosner, Bettina M.
Rainey, Frederick A.
Kroppenstedt, Reiner M.

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FEMS Microbiology Letters. 1997, 148(2), pp. 175-180. eISSN 0378-1097. Available under: doi: 10.1111/j.1574-6968.1997.tb10285.x

Zusammenfassung

Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of R. opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of R. ruber and Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

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Rhodococcus, Gordona, Pelobacter acetylenicus, acetylene hydratase, acetylene degradation

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ISO 690ROSNER, Bettina M., Frederick A. RAINEY, Reiner M. KROPPENSTEDT, Bernhard SCHINK, 1997. Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes. In: FEMS Microbiology Letters. 1997, 148(2), pp. 175-180. eISSN 0378-1097. Available under: doi: 10.1111/j.1574-6968.1997.tb10285.x
BibTex
@article{Rosner1997Acety-8258,
  year={1997},
  doi={10.1111/j.1574-6968.1997.tb10285.x},
  title={Acetylene degradation by new isolates of aerobic bacteria and comparison of acetylene hydratase enzymes},
  number={2},
  volume={148},
  journal={FEMS Microbiology Letters},
  pages={175--180},
  author={Rosner, Bettina M. and Rainey, Frederick A. and Kroppenstedt, Reiner M. and Schink, Bernhard}
}
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    <dcterms:abstract xml:lang="eng">Aerobic acetylene-degrading bacteria were isolated from soil samples. Two isolates were assigned to the species Rhodococcus opacus, two others to Rhodococcus ruber and Gordona sp. They were compared with known strains of aerobic acetylene-, cyanide-, or nitrile-utilizing bacteria. The acetylene hydratases of R. opacus could be measured in cell-free extracts only in the presence of a strong reductant like titanium(III) citrate. Expression of these enzymes was molybdenum-dependent. Acetylene hydratases in cell-free extracts of R. ruber and Gordona spp. did not require addition of reductants. No cross-reactivity could be found between cell-free extracts of any of these aerobic isolates and antibodies raised against the acetylene hydratase of the strictly anaerobic fermenting bacterium Pelobacter acetylenicus. These results show that acetylene hydratases are a biochemically heterogeneous group of enzymes.</dcterms:abstract>
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