Publikation:

Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation

Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2015

Autor:innen

Lühmann, Tessa
Jones, Gabriel
Gutmann, Marcus
Rybak, Jens-Christoph
Nickel, Joachim
Meinel, Lorenz

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
DOI (zitierfähiger Link)
https://doi.org/10.1021/acsbiomaterials.5b00236
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung

Sammlungen

Chemie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

ACS Biomaterials Science & Engineering. 2015, 1(9), pp. 740-746. eISSN 2373-9878. Available under: doi: 10.1021/acsbiomaterials.5b00236

Zusammenfassung

Presentation of therapeutic proteins on material surfaces is challenged by random immobilization chemistries through lysine or cysteine residues, typically leading to heterogeneous product outcome. Pharmaceutical quality standards warrant a controlled process ideally through site specific conjugation. Therefore, we deployed genetic codon expansion to engineer a propargyl-l-lysine (Plk)-modified FGF-2 analogue, enabling site-specific copper(I)-catalyzed azide alkyne cycloaddition (CuAAC). Site-specific decoration of Plk-FGF-2 to particles sparked cell proliferation of human osteosarcoma cells in a spatially controlled manner around the decorated carrier, rendering this approach instrumental for the future design of quality-improved bioinstructive scaffold outcome.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
540 Chemie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690LÜHMANN, Tessa, Gabriel JONES, Marcus GUTMANN, Jens-Christoph RYBAK, Joachim NICKEL, Marina RUBINI, Lorenz MEINEL, 2015. Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation. In: ACS Biomaterials Science & Engineering. 2015, 1(9), pp. 740-746. eISSN 2373-9878. Available under: doi: 10.1021/acsbiomaterials.5b00236
BibTex
@article{Luhmann2015Bioor-32668,
  year={2015},
  doi={10.1021/acsbiomaterials.5b00236},
  title={Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation},
  number={9},
  volume={1},
  journal={ACS Biomaterials Science & Engineering},
  pages={740--746},
  author={Lühmann, Tessa and Jones, Gabriel and Gutmann, Marcus and Rybak, Jens-Christoph and Nickel, Joachim and Rubini, Marina and Meinel, Lorenz}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/32668">
    <dc:creator>Rubini, Marina</dc:creator>
    <dc:contributor>Meinel, Lorenz</dc:contributor>
    <dc:contributor>Gutmann, Marcus</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-01-21T08:48:35Z</dc:date>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Gutmann, Marcus</dc:creator>
    <dc:contributor>Rubini, Marina</dc:contributor>
    <dc:language>eng</dc:language>
    <dcterms:issued>2015</dcterms:issued>
    <dcterms:title>Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation</dcterms:title>
    <dc:creator>Lühmann, Tessa</dc:creator>
    <dc:contributor>Nickel, Joachim</dc:contributor>
    <dc:creator>Meinel, Lorenz</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:contributor>Jones, Gabriel</dc:contributor>
    <dc:contributor>Lühmann, Tessa</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Rybak, Jens-Christoph</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-01-21T08:48:35Z</dcterms:available>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:abstract xml:lang="eng">Presentation of therapeutic proteins on material surfaces is challenged by random immobilization chemistries through lysine or cysteine residues, typically leading to heterogeneous product outcome. Pharmaceutical quality standards warrant a controlled process ideally through site specific conjugation. Therefore, we deployed genetic codon expansion to engineer a propargyl-l-lysine (Plk)-modified FGF-2 analogue, enabling site-specific copper(I)-catalyzed azide alkyne cycloaddition (CuAAC). Site-specific decoration of Plk-FGF-2 to particles sparked cell proliferation of human osteosarcoma cells in a spatially controlled manner around the decorated carrier, rendering this approach instrumental for the future design of quality-improved bioinstructive scaffold outcome.</dcterms:abstract>
    <dc:contributor>Rybak, Jens-Christoph</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/32668"/>
    <dc:creator>Nickel, Joachim</dc:creator>
    <dc:creator>Jones, Gabriel</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen