Crystal Structure of a Major Outer Membrane Protein from Thermus thermophilus HB27

Brosig, Alexander; Nesper, Jutta; Boos, Winfried; Welte, Wolfram; Diederichs, Kay

Crystal Structure of a Major Outer Membrane Protein from Thermus thermophilus HB27

Dateien

Brosig_etal_Crystal Structure.pdfGröße: 6.49 MBDownloads: 853

Datum

2009

Autor:innen

Nesper, Jutta

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Journal of Molecular Biology. 2009, 385(5), pp. 1445-1455. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/j.jmb.2008.12.003

Zusammenfassung

The thermophilic eubacterium Thermus thermophilus belongs to one of the oldest branches of evolution and has a multilayered cell envelope that differs from that of modern Gram-negative bacteria. Its outer membrane contains integral outer membrane proteins (OMPs), of which only a few are characterized. TtoA, a new p-barrel OMP, was identified by searching the genome sequence of strain HB27 for the presence of a C-terminal signature sequence. The structure of TtoA was determined to a resolution of 2.8 Å, representing the first crystal structure of an OMP from a thermophilic bacterium. TtoA consists of an eight-stranded p-barrel with a large extracellular part to which a divalent cation is bound. A five-stranded extracellular β-sheet protrudes out of the membrane-embedded transmembrane barrel and is stabilized by a disulfide bridge. The edge of this β-sheet forms crystal contacts that could mimic interactions with other proteins. In modem Gram-negative bacteria, the C-terminal signature sequence of OMPs is required for binding to an Omp85 family protein as a prerequisite for its assembly. We present hints that a similar assembly pathway exists in T. thermophilus by an in vitro binding assay, where unfolded TtoA binds to the Thermus Omp85 family protein TtOmp85, while a mutant without the signature sequence does not.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

outer membrane biogenesis, thermophile, T. thermophilus, TTC0834, TTC1475

Zitieren

ISO 690

BROSIG, Alexander, Jutta NESPER, Winfried BOOS, Wolfram WELTE, Kay DIEDERICHS, 2009. Crystal Structure of a Major Outer Membrane Protein from Thermus thermophilus HB27. In: Journal of Molecular Biology. 2009, 385(5), pp. 1445-1455. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/j.jmb.2008.12.003

BibTex

@article{Brosig2009-02-06Cryst-1125,
  year={2009},
  doi={10.1016/j.jmb.2008.12.003},
  title={Crystal Structure of a Major Outer Membrane Protein from Thermus thermophilus HB27},
  number={5},
  volume={385},
  issn={0022-2836},
  journal={Journal of Molecular Biology},
  pages={1445--1455},
  author={Brosig, Alexander and Nesper, Jutta and Boos, Winfried and Welte, Wolfram and Diederichs, Kay}
}

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Universitätsbibliographie

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