Publikation: Segment distributions of end-tethered polymers in a good solvent
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2006
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Physical Review Letters. 2006, 96, 107801. Available under: doi: 10.1103/PhysRevLett.96.107801
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We use confocal fluorescence microscopy to study the conformation of single DNA molecules endtethered to a solid substrate. The segment distribution ρ(z) measured for chains with contour lengths 15.4 μm ≤ L ≤ 59.4 μm as a function of the distance from the substrate z can be scaled onto a master curve depending only on the scaled distance z/Rg, in quantitative agreement with theoretical predictions for end-tethered polymers in a good solvent. The scaling of the radius of gyration Rg ~ L shows the presence of excluded-volume interactions between the charged DNA segments. Independent measurements of Rg from end-segment distributions are in good agreement with values obtained from the segment distributions and provide evidence that the radius of gyration of end-tethered chains in a good solvent is identical to that of the free chain.
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LEHNER, Roman, Juha KOOTA, Georg MARET, Thomas GISLER, 2006. Segment distributions of end-tethered polymers in a good solvent. In: Physical Review Letters. 2006, 96, 107801. Available under: doi: 10.1103/PhysRevLett.96.107801BibTex
@article{Lehner2006Segme-9434,
year={2006},
doi={10.1103/PhysRevLett.96.107801},
title={Segment distributions of end-tethered polymers in a good solvent},
volume={96},
journal={Physical Review Letters},
author={Lehner, Roman and Koota, Juha and Maret, Georg and Gisler, Thomas},
note={Article Number: 107801}
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<dcterms:abstract xml:lang="eng">We use confocal fluorescence microscopy to study the conformation of single DNA molecules endtethered to a solid substrate. The segment distribution ρ(z) measured for chains with contour lengths 15.4 μm ≤ L ≤ 59.4 μm as a function of the distance from the substrate z can be scaled onto a master curve depending only on the scaled distance z/Rg, in quantitative agreement with theoretical predictions for end-tethered polymers in a good solvent. The scaling of the radius of gyration Rg ~ L shows the presence of excluded-volume interactions between the charged DNA segments. Independent measurements of Rg from end-segment distributions are in good agreement with values obtained from the segment distributions and provide evidence that the radius of gyration of end-tethered chains in a good solvent is identical to that of the free chain.</dcterms:abstract>
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