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Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides

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2012

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Santner, Tobias
Micura, Ronald

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http://nbn-resolving.de/urn:nbn:de:bsz:352-205813
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https://doi.org/10.1039/C2CC35028A
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Chemical Communications. 2012, 48(79), pp. 9870-9872. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C2CC35028A

Zusammenfassung

We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2'-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2'-methylseleno-2'-deoxyuridine 5'-triphosphate.

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540 Chemie

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ISO 690SIEGMUND, Vanessa, Tobias SANTNER, Ronald MICURA, Andreas MARX, 2012. Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides. In: Chemical Communications. 2012, 48(79), pp. 9870-9872. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/C2CC35028A
BibTex
@article{Siegmund2012-10-11Scree-20581,
  year={2012},
  doi={10.1039/C2CC35028A},
  title={Screening mutant libraries of T7 RNA polymerase for candidates with increased acceptance of 2'-modified nucleotides},
  number={79},
  volume={48},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={9870--9872},
  author={Siegmund, Vanessa and Santner, Tobias and Micura, Ronald and Marx, Andreas}
}
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