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A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism

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2014

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Wojciechowski, Filip
Drescher, Malte
Hartig, Jörg S.

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http://nbn-resolving.de/urn:nbn:de:bsz:352-284300
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https://doi.org/10.1039/C4SC00440J
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Chemical Science. 2014, 5(7), pp. 2809-2818. ISSN 2041-6520. eISSN 2041-6539. Available under: doi: 10.1039/C4SC00440J

Zusammenfassung

The G-rich sequence d[(G4CT)3G4] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum. We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G4CT)3G4] displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G4CT)3G4] shows a so far unprecedented selectivity for K+ with even high concentrations of Na+ unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K+-concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.

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540 Chemie

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ISO 690REHM, Charlotte, Isabelle T. HOLDER, Andreas GROSS, Filip WOJCIECHOWSKI, Maximilian URBAN, Malte SINN, Malte DRESCHER, Jörg S. HARTIG, 2014. A bacterial DNA quadruplex with exceptional K+ selectivity and unique structural polymorphism. In: Chemical Science. 2014, 5(7), pp. 2809-2818. ISSN 2041-6520. eISSN 2041-6539. Available under: doi: 10.1039/C4SC00440J
BibTex
@article{Rehm2014bacte-28430,
  year={2014},
  doi={10.1039/C4SC00440J},
  title={A bacterial DNA quadruplex with exceptional K<sup>+</sup> selectivity and unique structural polymorphism},
  number={7},
  volume={5},
  issn={2041-6520},
  journal={Chemical Science},
  pages={2809--2818},
  author={Rehm, Charlotte and Holder, Isabelle T. and Groß, Andreas and Wojciechowski, Filip and Urban, Maximilian and Sinn, Malte and Drescher, Malte and Hartig, Jörg S.}
}
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    <dcterms:abstract xml:lang="eng">The G-rich sequence d[(G&lt;sub&gt;4&lt;/sub&gt;CT)&lt;sub&gt;3&lt;/sub&gt;G&lt;sub&gt;4&lt;/sub&gt;] was recently identified as a potential quadruplex-forming sequence associated with loci involved in antigenic variation in the human pathogen Treponema pallidum. We found this motif to be enriched in eubacterial genomes. Employing a combination of CD spectroscopy, EPR spectroscopy, analytical ultracentrifugation, and EMSA, we demonstrate that d[(G&lt;sub&gt;4&lt;/sub&gt;CT)&lt;sub&gt;3&lt;/sub&gt;G&lt;sub&gt;4&lt;/sub&gt;] &lt;/sub&gt;displays unique features among the many G-quadruplex-forming sequences studied so far. To our knowledge d[(G&lt;sub&gt;4&lt;/sub&gt;CT)&lt;sub&gt;3&lt;/sub&gt;G&lt;sub&gt;4&lt;/sub&gt;] shows a so far unprecedented selectivity for K&lt;sup&gt;+&lt;/sup&gt; with even high concentrations of Na&lt;sup&gt;+&lt;/sup&gt; unable to induce pronounced G-quadruplex formation. A remarkable continuous and complete transition from an anti-parallel, monomolecular structure into a tetrameric, parallel conformation is observed upon increasing K&lt;sup&gt;+&lt;/sup&gt;-concentrations. Furthermore we investigate the effects of cation selectivity, quadruplex loop composition and length as well as G-tract length on quadruplex conversion.</dcterms:abstract>
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