Pelorhabdus rhamnosifermentans gen. nov., sp. nov.,a strictly anaerobic rhamnose degrader from freshwater lake sediment

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2021
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Plugge, Caroline
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http://nbn-resolving.de/urn:nbn:de:bsz:352-2-99e0708d0xak0
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https://doi.org/10.1016/j.syapm.2021.126225
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Systematic and Applied Microbiology. Elsevier. 2021, 44(4), pp. 126225. ISSN 0723-2020. eISSN 1618-0984. Available under: doi: 10.1016/j.syapm.2021.126225
Zusammenfassung

A rhamnose-degrading bacterium, strain BoRhaAT, was isolated from profundal sediment of Lake Constance in agar dilution series with L-rhamnose as substrate and with a background lawn of Methanospirillum hungatei. The isolated strain was a motile rod that stained Gram positive. Growth was observed within a pH range of 4.0 - 7.5 and a temperature range of 15 to 30 °C. Fermentation products of rhamnose or glucose were acetate, propionate, ethanol, butyrate, and 1-propanol. The G+C content was 40.6% G+C. The dominant fatty acids are C16:1ω9c, i-C13:03OH, C16:0 and C17:1ω8c with 8-21% relative abundance. Polar lipids were glycolipids, phosphatidylethanolamine, phosphoaminolipid and other lipids, of which phosphatidylethanolamine was most abundant. The sequence of the 16S rRNA gene of the new isolate matches the sequence of its closest relative Anaerosporomusa subterranea to 92.4%. A comparison of the genome with this strain showed 60.2% genome-wide average amino acid identity (AAI), comparisons with other type strains showed a maximum of 62.7% AAI. Thus, the definition of a new genus is justified for which we propose the name Pelorhabdus. For strain BoRhaAT, we propose the name Pelorhabdus rhamnosifermentans gen. nov., sp. nov., with strain BoRhaAT (DSM 111565T = JCM 39158T) as the type strain.

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570 Biowissenschaften, Biologie
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Rhamnose, Anaerobic metabolism, Sugar fermentation
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ISO 690GRÄSSLE, Fabian, Caroline PLUGGE, Paolo FRANCHINI, Bernhard SCHINK, David SCHLEHECK, Nicolai MÜLLER, 2021. Pelorhabdus rhamnosifermentans gen. nov., sp. nov.,a strictly anaerobic rhamnose degrader from freshwater lake sediment. In: Systematic and Applied Microbiology. Elsevier. 2021, 44(4), pp. 126225. ISSN 0723-2020. eISSN 1618-0984. Available under: doi: 10.1016/j.syapm.2021.126225
BibTex
@article{Grale2021-07Pelor-54069,
  year={2021},
  doi={10.1016/j.syapm.2021.126225},
  title={Pelorhabdus rhamnosifermentans gen. nov., sp. nov.,a strictly anaerobic rhamnose degrader from freshwater lake sediment},
  number={4},
  volume={44},
  issn={0723-2020},
  journal={Systematic and Applied Microbiology},
  author={Gräßle, Fabian and Plugge, Caroline and Franchini, Paolo and Schink, Bernhard and Schleheck, David and Müller, Nicolai}
}
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    <dcterms:abstract xml:lang="eng">A rhamnose-degrading bacterium, strain BoRhaA&lt;sup&gt;T&lt;/sup&gt;, was isolated from profundal sediment of Lake Constance in agar dilution series with L-rhamnose as substrate and with a background lawn of Methanospirillum hungatei. The isolated strain was a motile rod that stained Gram positive. Growth was observed within a pH range of 4.0 - 7.5 and a temperature range of 15 to 30 °C. Fermentation products of rhamnose or glucose were acetate, propionate, ethanol, butyrate, and 1-propanol. The G+C content was 40.6% G+C. The dominant fatty acids are C&lt;sub&gt;16:1&lt;/sub&gt;ω9c, i-C&lt;sub&gt;13:03&lt;/sub&gt;OH, C&lt;sub&gt;16:0&lt;/sub&gt; and C&lt;sub&gt;17:1&lt;/sub&gt;ω8c with 8-21% relative abundance. Polar lipids were glycolipids, phosphatidylethanolamine, phosphoaminolipid and other lipids, of which phosphatidylethanolamine was most abundant. The sequence of the 16S rRNA gene of the new isolate matches the sequence of its closest relative Anaerosporomusa subterranea to 92.4%. A comparison of the genome with this strain showed 60.2% genome-wide average amino acid identity (AAI), comparisons with other type strains showed a maximum of 62.7% AAI. Thus, the definition of a new genus is justified for which we propose the name Pelorhabdus. For strain BoRhaA&lt;sup&gt;T&lt;/sup&gt;, we propose the name Pelorhabdus rhamnosifermentans gen. nov., sp. nov., with strain BoRhaA&lt;sup&gt;T&lt;/sup&gt; (DSM 111565&lt;sup&gt;T&lt;/sup&gt; = JCM 39158&lt;sup&gt;T&lt;/sup&gt;) as the type strain.</dcterms:abstract>
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