Publikation: Preparation of Clathrin-Coated Vesicles From Arabidopsis thaliana Seedlings
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2019
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Frontiers in Plant Science. 2019, 9, 1972. eISSN 1664-462X. Verfügbar unter: doi: 10.3389/fpls.2018.01972
Zusammenfassung
Clathrin coated vesicles (CCVs) mediate endocytosis of plasma membrane proteins and deliver their content to the endosomes for either subsequent recycling to the plasma membrane or transport to the vacuole for degradation. CCVs assemble also at the trans-Golgi network (TGN) and is responsible for the transport of proteins to other membranes. Oligomerization of clathrin and recruitment of adaptor protein complexes promote the budding and the release of CCVs. However, many of the details during plant CCV formation are not completely elucidated. The analysis of isolated CCVs is therefore important to better understand the formation of plant CCVs, their cargos and the regulation of clathrin-mediated transport processes. In this article, we describe an optimized method to isolate CCVs from Arabidopsis thaliana seedlings.
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570 Biowissenschaften, Biologie
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MOSESSO, Niccolo, Tobias BLÄSKE, Marie-Kristin NAGEL, Michael LAUMANN, Erika ISONO, 2019. Preparation of Clathrin-Coated Vesicles From Arabidopsis thaliana Seedlings. In: Frontiers in Plant Science. 2019, 9, 1972. eISSN 1664-462X. Verfügbar unter: doi: 10.3389/fpls.2018.01972BibTex
@article{Mosesso2019Prepa-44910,
year={2019},
doi={10.3389/fpls.2018.01972},
title={Preparation of Clathrin-Coated Vesicles From Arabidopsis thaliana Seedlings},
volume={9},
journal={Frontiers in Plant Science},
author={Mosesso, Niccolo and Bläske, Tobias and Nagel, Marie-Kristin and Laumann, Michael and Isono, Erika},
note={Article Number: 1972}
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<dcterms:abstract xml:lang="eng">Clathrin coated vesicles (CCVs) mediate endocytosis of plasma membrane proteins and deliver their content to the endosomes for either subsequent recycling to the plasma membrane or transport to the vacuole for degradation. CCVs assemble also at the trans-Golgi network (TGN) and is responsible for the transport of proteins to other membranes. Oligomerization of clathrin and recruitment of adaptor protein complexes promote the budding and the release of CCVs. However, many of the details during plant CCV formation are not completely elucidated. The analysis of isolated CCVs is therefore important to better understand the formation of plant CCVs, their cargos and the regulation of clathrin-mediated transport processes. In this article, we describe an optimized method to isolate CCVs from Arabidopsis thaliana seedlings.</dcterms:abstract>
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