Publikation: Soluble Major Histocompatibility Complex-Peptide Octamers with Impaired CD8 Binding Selectively Induce Fas-dependent Apoptosis
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Fluorescence-labeled soluble major histocompatibility complex class I-peptide "tetramers" constitute a powerful tool to detect and isolate antigen-specific CD8+ T cells by flow cytometry. Conventional "tetramers" are prepared by refolding of heavy and light chains with a specific peptide, enzymatic biotinylation at an added C-terminal biotinylation sequence, and "tetramerization" by reaction with phycoerythrin- or allophycocyanin-labeled avidin derivatives. We show here that such preparations are heterogeneous and describe a new procedure that allows the preparation of homogeneous tetra- or octameric major histocompatibility complex-peptide complexes. These compounds were tested on T1 cytotoxic T lymphocytes (CTLs), which recognize the Plasmodium berghei circumsporzoite peptide 252-260 (SYIPSAEKI) containing photoreactive 4-azidobenzoic acid on Lys259 in the context of H-2Kd. We report that mutation of the CD8 binding site of Kd greatly impairs the binding of tetrameric but not octameric or multimeric Kd-PbCS(ABA) complexes to CTLs. This mutation abolishes the ability of the octamer to elicit significant phosphorylation of CD3, intracellular calcium mobilization, and CTL degranulation. Remarkably, however, this octamer efficiently activates CTLs for Fas (CD95)-dependent apoptosis.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
GUILLAUME, Philippe, Daniel F. LEGLER, Nicole BOUCHERON, Marie-Agnès DOUCEY, Jean-Charles CEROTTINI, Immanuel F. LUESCHER, 2003. Soluble Major Histocompatibility Complex-Peptide Octamers with Impaired CD8 Binding Selectively Induce Fas-dependent Apoptosis. In: Journal of Biological Chemistry. 2003, 278(7), pp. 4500-4509. ISSN 0021-9258. eISSN 1083-351X. Available under: doi: 10.1074/jbc.M208863200BibTex
@article{Guillaume2003Solub-36628, year={2003}, doi={10.1074/jbc.M208863200}, title={Soluble Major Histocompatibility Complex-Peptide Octamers with Impaired CD8 Binding Selectively Induce Fas-dependent Apoptosis}, number={7}, volume={278}, issn={0021-9258}, journal={Journal of Biological Chemistry}, pages={4500--4509}, author={Guillaume, Philippe and Legler, Daniel F. and Boucheron, Nicole and Doucey, Marie-Agnès and Cerottini, Jean-Charles and Luescher, Immanuel F.} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/36628"> <dc:contributor>Cerottini, Jean-Charles</dc:contributor> <dc:creator>Legler, Daniel F.</dc:creator> <dc:creator>Luescher, Immanuel F.</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/36628/1/Guillaume_0-366204.pdf"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/36628/1/Guillaume_0-366204.pdf"/> <dc:creator>Guillaume, Philippe</dc:creator> <dc:contributor>Guillaume, Philippe</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-12T09:43:41Z</dcterms:available> <dc:creator>Boucheron, Nicole</dc:creator> <dc:contributor>Boucheron, Nicole</dc:contributor> <dc:contributor>Legler, Daniel F.</dc:contributor> <dc:language>eng</dc:language> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Doucey, Marie-Agnès</dc:contributor> <dcterms:abstract xml:lang="eng">Fluorescence-labeled soluble major histocompatibility complex class I-peptide "tetramers" constitute a powerful tool to detect and isolate antigen-specific CD8<sup>+</sup> T cells by flow cytometry. Conventional "tetramers" are prepared by refolding of heavy and light chains with a specific peptide, enzymatic biotinylation at an added C-terminal biotinylation sequence, and "tetramerization" by reaction with phycoerythrin- or allophycocyanin-labeled avidin derivatives. We show here that such preparations are heterogeneous and describe a new procedure that allows the preparation of homogeneous tetra- or octameric major histocompatibility complex-peptide complexes. These compounds were tested on T1 cytotoxic T lymphocytes (CTLs), which recognize the Plasmodium berghei circumsporzoite peptide 252-260 (SYIPSAEKI) containing photoreactive 4-azidobenzoic acid on Lys<sup>259</sup> in the context of H-2K<sup>d</sup>. We report that mutation of the CD8 binding site of K<sup>d</sup> greatly impairs the binding of tetrameric but not octameric or multimeric K<sup>d</sup>-PbCS(ABA) complexes to CTLs. This mutation abolishes the ability of the octamer to elicit significant phosphorylation of CD3, intracellular calcium mobilization, and CTL degranulation. Remarkably, however, this octamer efficiently activates CTLs for Fas (CD95)-dependent apoptosis.</dcterms:abstract> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-12T09:43:41Z</dc:date> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:rights>terms-of-use</dc:rights> <dcterms:issued>2003</dcterms:issued> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/36628"/> <dc:creator>Doucey, Marie-Agnès</dc:creator> <dc:creator>Cerottini, Jean-Charles</dc:creator> <dc:contributor>Luescher, Immanuel F.</dc:contributor> <dcterms:title>Soluble Major Histocompatibility Complex-Peptide Octamers with Impaired CD8 Binding Selectively Induce Fas-dependent Apoptosis</dcterms:title> </rdf:Description> </rdf:RDF>