@article{Philipp2000disti-7506,
  year={2000},
  doi={10.1007/s002039900112},
  title={Two distinct pathways for anaerobic degradation of aromatic compounds in the denitrifying bacterium Thauera aromatica strain AR-1},
  number={2},
  volume={173},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={91--96},
  author={Philipp, Bodo and Schink, Bernhard}
}

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    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 173 (2000), 2, pp. 91-96</dcterms:bibliographicCitation>
    <dcterms:abstract xml:lang="eng">Denitrifying bacteria degrade many different aromatic compounds anaerobically via the well-described benzoyl-CoA pathway. We have shown recently that the denitrifiers Azoarcus anaerobius and Thauera aromatica strain AR-1 use a different pathway for anaerobic degradation of resorcinol (1,3-dihydroxybenzene) and 3,5-dihydroxybenzoate, respectively. Both substrates are converted to hydroxyhydroquinone (1,2,4-trihydroxybenzene). In the membrane fraction of T. aromatica strain AR-1 cells grown with 3,5-dihydroxybenzoate, a hydroxyhydroquinone-dehydrogenating activity of 74 nmol min 1(mg protein) 1 was found. This activity was significantly lower in benzoate-grown cells. Benzoate-grown cells were not induced for degradation of 3,5-dihydroxybenzoate, and cells grown with 3,5-dihydroxybenzoate degraded benzoate only at a very low rate. With a substrate mixture of benzoate plus 3,5-dihydroxybenzoate, the cells showed diauxic growth. Benzoate was degraded first, while complete degradation of 3,5-dihydroxybenzoate occurred only after a long lag phase. The 3,5-dihydroxybenzoate-oxidizing and the hydroxyhydroquinone-dehydrogenating activities were fully induced only during 3,5-dihydroxybenzoate degradation. Synthesis of benzoyl-CoA reductase appeared to be significantly lower in 3,5-dihydroxybenzoate-grown cells as shown by immunoblotting. These results confirm that T. aromatica strain AR-1 harbors, in addition to the benzoyl-CoA pathway, a second, mechanistically distinct pathway for anaerobic degradation of aromatic compounds. This pathway is inducible and subject to catabolite repression by benzoate.</dcterms:abstract>
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    <dc:contributor>Schink, Bernhard</dc:contributor>
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    <dc:contributor>Philipp, Bodo</dc:contributor>
    <dc:creator>Philipp, Bodo</dc:creator>
    <dc:creator>Schink, Bernhard</dc:creator>
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