De novo sequencing of peptides on single resin beads by MALDI-FTICR tandem mass spectrometry

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Journal of the American Society for Mass Spectrometry. 2010, 21(2), pp. 215-219. ISSN 1044-0305. eISSN 1879-1123. Available under: doi: 10.1016/j.jasms.2009.10.004
Zusammenfassung

An efficient approach in combinatorial chemistry is the synthesis of one-bead-one-compound peptide libraries. In contrast to synthesis and functional screening, which is performed in a largely automated manner, structure determination has been frequently laborious and time-consuming. Here we report an approach for de novo sequencing of peptides on single beads by matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) tandem mass spectrometry, using a resin with a photolinker for solid-phase peptide synthesis. Upon sorting out single beads, an efficient sample preparation on the MALDI target was developed that enables fragmentation upon irradiation of the bead-matrix mixture with the ultraviolet (UV)-MALDI laser, with enhanced yield of sequence-specific fragment ions at increased laser energy. This approach is illustrated by sequence determinations of two peptides from a library with sequences varying in a single amino acid; the feasibility with tandem-MS procedures and fragment ion assignment was ascertained by sustained off-resonance irradiation/collision induced dissociation (SORI/CID) and infrared multiphoton dissociation (IRMPD) fragmentation.

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ISO 690SEMMLER, Angelika, Reinhold WEBER, Michael PRZYBYLSKI, Valentin WITTMANN, 2010. De novo sequencing of peptides on single resin beads by MALDI-FTICR tandem mass spectrometry. In: Journal of the American Society for Mass Spectrometry. 2010, 21(2), pp. 215-219. ISSN 1044-0305. eISSN 1879-1123. Available under: doi: 10.1016/j.jasms.2009.10.004
BibTex
@article{Semmler2010seque-9644,
  year={2010},
  doi={10.1016/j.jasms.2009.10.004},
  title={De novo sequencing of peptides on single resin beads by MALDI-FTICR tandem mass spectrometry},
  number={2},
  volume={21},
  issn={1044-0305},
  journal={Journal of the American Society for Mass Spectrometry},
  pages={215--219},
  author={Semmler, Angelika and Weber, Reinhold and Przybylski, Michael and Wittmann, Valentin}
}
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