Biological applications of fluorescence lifetime imaging beyond microscopy

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2010
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Akers, Walter J.
Berezin, Mikhail Y.
Lee, Hyeran
Guo, Kevin
Almutairi, Adah
Fréchet, Jean M. J.
Achilefu, Samuel
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ACHILEFU, Samuel, ed. and others. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications II : 25 - 27 January 2010, San Francisco, California, United States. Bellingham, Washington: SPIE, 2010, 757612. Proceedings of SPIE. 7576. eISSN 0277-786X. ISBN 978-0-8194-7972-3. Available under: doi: 10.1117/12.849453
Zusammenfassung

Fluorescence lifetime is a relatively new contrast mechanism for optical imaging in living subjects that relies on intrinsic properties of fluorophores rather than concentration dependent intensity. Drawing upon the success of fluorescence lifetime imaging microscopy (FLIM) for investigation of protein-protein interactions and intracellular physiology, in vivo fluorescence lifetime imaging (FLI) promises to dramatically increase the utility of fluorescencebased imaging in preclinical and clinical applications. Intrinsic fluorescence lifetime measurements in living tissues can distinguish pathologies such as cancer from healthy tissue. Unfortunately, intrinsic FLT contrast is limited to superficial measurements. Conventional intensity-based agents have been reported for measuring these phenomena in vitro, but translation into living animals is difficult due to optical properties of tissues. For this reason, contrast agents that can be detected in the near infrared (NIR) wavelengths are being developed by our lab and others to enhance the capabilities of this modality. FLT is less affected by concentration and thus is better for detecting small changes in physiology, as long as sufficient fluorescence signal can be measured. FLT can also improve localization of signals for improved deep tissue imaging. Examples of the utility of exogenous contrast agents will be discussed, including applications in monitoring physiologic functions, controlled drug release and cancer biology. Instrumentation for FLI will also be discussed, including planar and diffuse optical imaging in time and frequency domains. Future applications will also be discussed that are being developed in this exciting field that complement other optical modalities.

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540 Chemie
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Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications II, 25. Jan. 2010 - 27. Jan. 2010, San Francisco, California
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ISO 690AKERS, Walter J., Mikhail Y. BEREZIN, Hyeran LEE, Kevin GUO, Adah ALMUTAIRI, Jean M. J. FRÉCHET, Georg FISCHER, Ewald DALTROZZO, Samuel ACHILEFU, 2010. Biological applications of fluorescence lifetime imaging beyond microscopy. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications II. San Francisco, California, 25. Jan. 2010 - 27. Jan. 2010. In: ACHILEFU, Samuel, ed. and others. Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications II : 25 - 27 January 2010, San Francisco, California, United States. Bellingham, Washington: SPIE, 2010, 757612. Proceedings of SPIE. 7576. eISSN 0277-786X. ISBN 978-0-8194-7972-3. Available under: doi: 10.1117/12.849453
BibTex
@inproceedings{Akers2010-02-17Biolo-53882,
  year={2010},
  doi={10.1117/12.849453},
  title={Biological applications of fluorescence lifetime imaging beyond microscopy},
  number={7576},
  isbn={978-0-8194-7972-3},
  publisher={SPIE},
  address={Bellingham, Washington},
  series={Proceedings of SPIE},
  booktitle={Reporters, markers, dyes, nanoparticles, and molecular probes for biomedical applications II : 25 - 27 January 2010, San Francisco, California, United States},
  editor={Achilefu, Samuel},
  author={Akers, Walter J. and Berezin, Mikhail Y. and Lee, Hyeran and Guo, Kevin and Almutairi, Adah and Fréchet, Jean M. J. and Fischer, Georg and Daltrozzo, Ewald and Achilefu, Samuel},
  note={Article Number: 757612}
}
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