Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve

Lade...
Vorschaubild
Dateien
Bodrikov_2-b9j5jgorzzbh5.pdf
Bodrikov_2-b9j5jgorzzbh5.pdfGröße: 778.22 KBDownloads: 658
Datum
2017
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

This study explored why lesioned retinal ganglion cell (RGC) axons regenerate successfully in the zebrafish optic nerve despite the presence of Rtn4b, the homologue of the rat neurite growth inhibitor RTN4-A/Nogo-A. Rat Nogo-A and zebrafish Rtn4b possess characteristic motifs (M1-4) in the Nogo-A-specific region, which contains delta20, the most inhibitory region of rat Nogo-A. To determine whether zebrafish M1-4 is inhibitory as rat M1-4 and Nogo-A delta20, proteins were recombinantly expressed and used as substrates for zebrafish single cell RGCs, mouse hippocampal neurons and goldfish, zebrafish and chick retinal explants. When offered as homogenous substrates, neurites of hippocampal neurons and of zebrafish single cell RGCs were inhibited by zebrafish M1-4, rat M1-4, and Nogo-A delta20. Neurite length increased when zebrafish single cell RGCs were treated with receptor-type-specific antagonists and, respectively, with morpholinos (MO) against S1PR2 and S1PR5a-which represent candidate zebrafish Nogo-A receptors. In a stripe assay, however, where M1-4 lanes alternate with polylysine-(Plys)-only lanes, RGC axons from goldfish, zebrafish, and chick retinal explants avoided rat M1-4 but freely crossed zebrafish M1-4 lanes-suggesting that zebrafish M1-4 is growth permissive and less inhibitory than rat M1-4. Moreover, immunostainings and dot blots of optic nerve and myelin showed that expression of Rtn4b is very low in tissue and myelin at 3-5 days after lesion when axons regenerate. Thus, Rtn4b seems to represent no major obstacle for axon regeneration in vivo because it is less inhibitory for RGC axons from retina explants, and because of its low abundance.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690BODRIKOV, Vsevolod, Cornelia WELTE, Marianne F. WIECHERS, Markus WESCHENFELDER, Gurjot KAUR, Aleksandra SHYPITSYNA, Alejandro PINZON-OLEJUA, Martin BASTMEYER, Claudia STÜRMER, 2017. Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve. In: The Journal of Comparative Neurology. 2017, 525(14), pp. 2991-3009. ISSN 0021-9967. eISSN 1096-9861. Available under: doi: 10.1002/cne.24253
BibTex
@article{Bodrikov2017-10-01Subst-40496,
  year={2017},
  doi={10.1002/cne.24253},
  title={Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve},
  number={14},
  volume={525},
  issn={0021-9967},
  journal={The Journal of Comparative Neurology},
  pages={2991--3009},
  author={Bodrikov, Vsevolod and Welte, Cornelia and Wiechers, Marianne F. and Weschenfelder, Markus and Kaur, Gurjot and Shypitsyna, Aleksandra and Pinzon-Olejua, Alejandro and Bastmeyer, Martin and Stürmer, Claudia}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/40496">
    <dc:contributor>Pinzon-Olejua, Alejandro</dc:contributor>
    <dc:contributor>Bodrikov, Vsevolod</dc:contributor>
    <dc:contributor>Shypitsyna, Aleksandra</dc:contributor>
    <dc:contributor>Weschenfelder, Markus</dc:contributor>
    <dc:creator>Stürmer, Claudia</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/40496"/>
    <dc:creator>Bastmeyer, Martin</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Stürmer, Claudia</dc:contributor>
    <dc:contributor>Bastmeyer, Martin</dc:contributor>
    <dc:creator>Welte, Cornelia</dc:creator>
    <dcterms:abstract xml:lang="eng">This study explored why lesioned retinal ganglion cell (RGC) axons regenerate successfully in the zebrafish optic nerve despite the presence of Rtn4b, the homologue of the rat neurite growth inhibitor RTN4-A/Nogo-A. Rat Nogo-A and zebrafish Rtn4b possess characteristic motifs (M1-4) in the Nogo-A-specific region, which contains delta20, the most inhibitory region of rat Nogo-A. To determine whether zebrafish M1-4 is inhibitory as rat M1-4 and Nogo-A delta20, proteins were recombinantly expressed and used as substrates for zebrafish single cell RGCs, mouse hippocampal neurons and goldfish, zebrafish and chick retinal explants. When offered as homogenous substrates, neurites of hippocampal neurons and of zebrafish single cell RGCs were inhibited by zebrafish M1-4, rat M1-4, and Nogo-A delta20. Neurite length increased when zebrafish single cell RGCs were treated with receptor-type-specific antagonists and, respectively, with morpholinos (MO) against S1PR2 and S1PR5a-which represent candidate zebrafish Nogo-A receptors. In a stripe assay, however, where M1-4 lanes alternate with polylysine-(Plys)-only lanes, RGC axons from goldfish, zebrafish, and chick retinal explants avoided rat M1-4 but freely crossed zebrafish M1-4 lanes-suggesting that zebrafish M1-4 is growth permissive and less inhibitory than rat M1-4. Moreover, immunostainings and dot blots of optic nerve and myelin showed that expression of Rtn4b is very low in tissue and myelin at 3-5 days after lesion when axons regenerate. Thus, Rtn4b seems to represent no major obstacle for axon regeneration in vivo because it is less inhibitory for RGC axons from retina explants, and because of its low abundance.</dcterms:abstract>
    <dc:contributor>Wiechers, Marianne F.</dc:contributor>
    <dc:creator>Shypitsyna, Aleksandra</dc:creator>
    <dc:creator>Pinzon-Olejua, Alejandro</dc:creator>
    <dcterms:issued>2017-10-01</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/40496/1/Bodrikov_2-b9j5jgorzzbh5.pdf"/>
    <dc:creator>Wiechers, Marianne F.</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-11-07T09:27:41Z</dcterms:available>
    <dc:rights>terms-of-use</dc:rights>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-11-07T09:27:41Z</dc:date>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/40496/1/Bodrikov_2-b9j5jgorzzbh5.pdf"/>
    <dc:creator>Kaur, Gurjot</dc:creator>
    <dc:creator>Bodrikov, Vsevolod</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:title>Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve</dcterms:title>
    <dc:creator>Weschenfelder, Markus</dc:creator>
    <dc:contributor>Welte, Cornelia</dc:contributor>
    <dc:contributor>Kaur, Gurjot</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen