Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve

Loading...
Thumbnail Image
Date
2017
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
DOI (citable link)
ArXiv-ID
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published
Published in
The Journal of Comparative Neurology ; 525 (2017), 14. - pp. 2991-3009. - ISSN 0021-9967. - eISSN 1096-9861
Abstract
This study explored why lesioned retinal ganglion cell (RGC) axons regenerate successfully in the zebrafish optic nerve despite the presence of Rtn4b, the homologue of the rat neurite growth inhibitor RTN4-A/Nogo-A. Rat Nogo-A and zebrafish Rtn4b possess characteristic motifs (M1-4) in the Nogo-A-specific region, which contains delta20, the most inhibitory region of rat Nogo-A. To determine whether zebrafish M1-4 is inhibitory as rat M1-4 and Nogo-A delta20, proteins were recombinantly expressed and used as substrates for zebrafish single cell RGCs, mouse hippocampal neurons and goldfish, zebrafish and chick retinal explants. When offered as homogenous substrates, neurites of hippocampal neurons and of zebrafish single cell RGCs were inhibited by zebrafish M1-4, rat M1-4, and Nogo-A delta20. Neurite length increased when zebrafish single cell RGCs were treated with receptor-type-specific antagonists and, respectively, with morpholinos (MO) against S1PR2 and S1PR5a-which represent candidate zebrafish Nogo-A receptors. In a stripe assay, however, where M1-4 lanes alternate with polylysine-(Plys)-only lanes, RGC axons from goldfish, zebrafish, and chick retinal explants avoided rat M1-4 but freely crossed zebrafish M1-4 lanes-suggesting that zebrafish M1-4 is growth permissive and less inhibitory than rat M1-4. Moreover, immunostainings and dot blots of optic nerve and myelin showed that expression of Rtn4b is very low in tissue and myelin at 3-5 days after lesion when axons regenerate. Thus, Rtn4b seems to represent no major obstacle for axon regeneration in vivo because it is less inhibitory for RGC axons from retina explants, and because of its low abundance.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690BODRIKOV, Vsevolod, Cornelia WELTE, Marianne F. WIECHERS, Markus WESCHENFELDER, Gurjot KAUR, Aleksandra SHYPITSYNA, Alejandro PINZON-OLEJUA, Martin BASTMEYER, Claudia STÜRMER, 2017. Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve. In: The Journal of Comparative Neurology. 525(14), pp. 2991-3009. ISSN 0021-9967. eISSN 1096-9861. Available under: doi: 10.1002/cne.24253
BibTex
@article{Bodrikov2017-10-01Subst-40496,
  year={2017},
  doi={10.1002/cne.24253},
  title={Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve},
  number={14},
  volume={525},
  issn={0021-9967},
  journal={The Journal of Comparative Neurology},
  pages={2991--3009},
  author={Bodrikov, Vsevolod and Welte, Cornelia and Wiechers, Marianne F. and Weschenfelder, Markus and Kaur, Gurjot and Shypitsyna, Aleksandra and Pinzon-Olejua, Alejandro and Bastmeyer, Martin and Stürmer, Claudia}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/40496">
    <dc:contributor>Pinzon-Olejua, Alejandro</dc:contributor>
    <dc:contributor>Bodrikov, Vsevolod</dc:contributor>
    <dc:contributor>Shypitsyna, Aleksandra</dc:contributor>
    <dc:contributor>Weschenfelder, Markus</dc:contributor>
    <dc:creator>Stürmer, Claudia</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/40496"/>
    <dc:creator>Bastmeyer, Martin</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Stürmer, Claudia</dc:contributor>
    <dc:contributor>Bastmeyer, Martin</dc:contributor>
    <dc:creator>Welte, Cornelia</dc:creator>
    <dcterms:abstract xml:lang="eng">This study explored why lesioned retinal ganglion cell (RGC) axons regenerate successfully in the zebrafish optic nerve despite the presence of Rtn4b, the homologue of the rat neurite growth inhibitor RTN4-A/Nogo-A. Rat Nogo-A and zebrafish Rtn4b possess characteristic motifs (M1-4) in the Nogo-A-specific region, which contains delta20, the most inhibitory region of rat Nogo-A. To determine whether zebrafish M1-4 is inhibitory as rat M1-4 and Nogo-A delta20, proteins were recombinantly expressed and used as substrates for zebrafish single cell RGCs, mouse hippocampal neurons and goldfish, zebrafish and chick retinal explants. When offered as homogenous substrates, neurites of hippocampal neurons and of zebrafish single cell RGCs were inhibited by zebrafish M1-4, rat M1-4, and Nogo-A delta20. Neurite length increased when zebrafish single cell RGCs were treated with receptor-type-specific antagonists and, respectively, with morpholinos (MO) against S1PR2 and S1PR5a-which represent candidate zebrafish Nogo-A receptors. In a stripe assay, however, where M1-4 lanes alternate with polylysine-(Plys)-only lanes, RGC axons from goldfish, zebrafish, and chick retinal explants avoided rat M1-4 but freely crossed zebrafish M1-4 lanes-suggesting that zebrafish M1-4 is growth permissive and less inhibitory than rat M1-4. Moreover, immunostainings and dot blots of optic nerve and myelin showed that expression of Rtn4b is very low in tissue and myelin at 3-5 days after lesion when axons regenerate. Thus, Rtn4b seems to represent no major obstacle for axon regeneration in vivo because it is less inhibitory for RGC axons from retina explants, and because of its low abundance.</dcterms:abstract>
    <dc:contributor>Wiechers, Marianne F.</dc:contributor>
    <dc:creator>Shypitsyna, Aleksandra</dc:creator>
    <dc:creator>Pinzon-Olejua, Alejandro</dc:creator>
    <dcterms:issued>2017-10-01</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/40496/1/Bodrikov_2-b9j5jgorzzbh5.pdf"/>
    <dc:creator>Wiechers, Marianne F.</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-11-07T09:27:41Z</dcterms:available>
    <dc:rights>terms-of-use</dc:rights>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-11-07T09:27:41Z</dc:date>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/40496/1/Bodrikov_2-b9j5jgorzzbh5.pdf"/>
    <dc:creator>Kaur, Gurjot</dc:creator>
    <dc:creator>Bodrikov, Vsevolod</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:title>Substrate properties of zebrafish Rtn4b/Nogo and axon regeneration in the zebrafish optic nerve</dcterms:title>
    <dc:creator>Weschenfelder, Markus</dc:creator>
    <dc:contributor>Welte, Cornelia</dc:contributor>
    <dc:contributor>Kaur, Gurjot</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed