Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold

Sauter, Katharina B.; Marx, Andreas

Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold

Files

Sauter_167259.pdf(149.28 KB)

Date

2006

Authors

Sauter, Katharina B.

Marx, Andreas

Publication type

Journal article

Published in

Angewandte Chemie International Edition ; 45 (2006), 45. - pp. 7633-7635. - ISSN 1433-7851

Abstract

Thermostable DNA polymerases normally exhibit no significant intrinsic activities to reverse transcribe RNA into DNA. We show that enzymes with significantly increased reverse transcription ability can be generated by directed evolution.

Subject (DDC)

540 Chemistry

Keywords

directed evolution,DNA polymerase,DNA recognition,polymerase chain reaction,reverse transcriptase

Cite This

ISO 690

SAUTER, Katharina B., Andreas MARX, 2006. Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold. In: Angewandte Chemie International Edition. 45(45), pp. 7633-7635. ISSN 1433-7851. Available under: doi: 10.1002/anie.200602772

BibTex

@article{Sauter2006-11-20Evolv-16725,
  year={2006},
  doi={10.1002/anie.200602772},
  title={Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold},
  number={45},
  volume={45},
  issn={1433-7851},
  journal={Angewandte Chemie International Edition},
  pages={7633--7635},
  author={Sauter, Katharina B. and Marx, Andreas}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/16725">
    <dc:language>eng</dc:language>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/16725"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16725/2/Sauter_167259.pdf"/>
    <dc:creator>Sauter, Katharina B.</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-11-16T09:56:19Z</dcterms:available>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dcterms:abstract xml:lang="eng">Thermostable DNA polymerases normally exhibit no significant intrinsic activities to reverse transcribe RNA into DNA. We show that enzymes with significantly increased reverse transcription ability can be generated by directed evolution.</dcterms:abstract>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-11-16T09:56:19Z</dc:date>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/16725/2/Sauter_167259.pdf"/>
    <dcterms:issued>2006-11-20</dcterms:issued>
    <dc:contributor>Sauter, Katharina B.</dc:contributor>
    <dcterms:title>Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold</dcterms:title>
    <dc:creator>Marx, Andreas</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:bibliographicCitation>Publ. in: Angewandte Chemie International Edition ; 45 (2006), 45. - pp. 7633–7635</dcterms:bibliographicCitation>
  </rdf:Description>
</rdf:RDF>

Bibliography of Konstanz

Yes