@article{Lenkeit2020Disco-52578,
  year={2020},
  doi={10.1093/nar/gkaa1102},
  title={Discovery and characterization of a fourth class of guanidine riboswitches},
  number={22},
  volume={48},
  issn={0305-1048},
  journal={Nucleic Acids Research},
  pages={12889--12899},
  author={Lenkeit, Felina and Eckert, Iris and Hartig, Jörg S. and Weinberg, Zasha}
}

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    <dcterms:abstract xml:lang="eng">Riboswitches are RNAs that specifically sense a small molecule and regulate genes accordingly. The recent discovery of guanidine-binding riboswitches revealed the biological significance of this compound, and uncovered genes related to its biology. For example, certain sugE genes encode guanidine exporters and are activated by the riboswitches to reduce toxic levels of guanidine in the cell. In order to study guanidine biology and riboswitches, we applied a bioinformatics strategy for discovering additional guanidine riboswitches by searching for new candidate motifs associated with sugE genes. Based on in vitro and in vivo experiments, we determined that one of our six best candidates is a new structural class of guanidine riboswitches. The expression of a genetic reporter was induced 80-fold in response to addition of 5 mM guanidine in Staphylococcus aureus. This new class, called the guanidine-IV riboswitch, reveals additional guanidine-associated protein domains that are extremely rarely or never associated with previously established guanidine riboswitches. Among these protein domains are two transporter families that are structurally distinct from SugE, and could represent novel types of guanidine exporters. These results establish a new metabolite-binding RNA, further validate a bioinformatics method for finding riboswitches and suggest substrate specificities for as-yet uncharacterized transporter proteins.</dcterms:abstract>
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    <dc:creator>Eckert, Iris</dc:creator>
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    <dc:contributor>Lenkeit, Felina</dc:contributor>
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    <dc:creator>Lenkeit, Felina</dc:creator>
    <dc:creator>Hartig, Jörg S.</dc:creator>
    <dc:contributor>Eckert, Iris</dc:contributor>
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    <dcterms:title>Discovery and characterization of a fourth class of guanidine riboswitches</dcterms:title>
    <dc:rights>Attribution 4.0 International</dc:rights>
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    <dc:creator>Weinberg, Zasha</dc:creator>
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    <dc:contributor>Weinberg, Zasha</dc:contributor>
    <dc:contributor>Hartig, Jörg S.</dc:contributor>
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