Publikation:

The ubiquitin-like modifier FAT10 interferes with SUMO activation

Lade...
Vorschaubild

Dateien

Aichem_2-bk0hoidsar8l4.pdf
Aichem_2-bk0hoidsar8l4.pdfGröße: 2.16 MBDownloads: 420

Datum

2019

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Link zur Lizenz

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Gold
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Nature communications. 2019, 10, 4452. eISSN 2041-1723. Available under: doi: 10.1038/s41467-019-12430-z

Zusammenfassung

The covalent attachment of the cytokine-inducible ubiquitin-like modifier HLA-F adjacent transcript 10 (FAT10) to hundreds of substrate proteins leads to their rapid degradation by the 26 S proteasome independently of ubiquitylation. Here, we identify another function of FAT10, showing that it interferes with the activation of SUMO1/2/3 in vitro and down-regulates SUMO conjugation and the SUMO-dependent formation of promyelocytic leukemia protein (PML) bodies in cells. Mechanistically, we show that FAT10 directly binds to and impedes the activity of the heterodimeric SUMO E1 activating enzyme AOS1/UBA2 by competing very efficiently with SUMO for activation and thioester formation. Nevertheless, activation of FAT10 by AOS1/UBA2 does not lead to covalent conjugation of FAT10 with substrate proteins which relies on its cognate E1 enzyme UBA6. Hence, we report that one ubiquitin-like modifier (FAT10) inhibits the conjugation and function of another ubiquitin-like modifier (SUMO) by impairing its activation.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690AICHEM, Annette, Carolin SAILER, Stella RYU, Nicola CATONE, Nicolas STANKOVIC-VALENTIN, Gunter SCHMIDTKE, Frauke MELCHIOR, Florian STENGEL, Marcus GRÖTTRUP, 2019. The ubiquitin-like modifier FAT10 interferes with SUMO activation. In: Nature communications. 2019, 10, 4452. eISSN 2041-1723. Available under: doi: 10.1038/s41467-019-12430-z
BibTex
@article{Aichem2019-10-01ubiqu-47200,
  year={2019},
  doi={10.1038/s41467-019-12430-z},
  title={The ubiquitin-like modifier FAT10 interferes with SUMO activation},
  volume={10},
  journal={Nature communications},
  author={Aichem, Annette and Sailer, Carolin and Ryu, Stella and Catone, Nicola and Stankovic-Valentin, Nicolas and Schmidtke, Gunter and Melchior, Frauke and Stengel, Florian and Gröttrup, Marcus},
  note={Article Number: 4452}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/47200">
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47200/3/Aichem_2-bk0hoidsar8l4.pdf"/>
    <dc:contributor>Stengel, Florian</dc:contributor>
    <dc:contributor>Aichem, Annette</dc:contributor>
    <dcterms:abstract xml:lang="eng">The covalent attachment of the cytokine-inducible ubiquitin-like modifier HLA-F adjacent transcript 10 (FAT10) to hundreds of substrate proteins leads to their rapid degradation by the 26 S proteasome independently of ubiquitylation. Here, we identify another function of FAT10, showing that it interferes with the activation of SUMO1/2/3 in vitro and down-regulates SUMO conjugation and the SUMO-dependent formation of promyelocytic leukemia protein (PML) bodies in cells. Mechanistically, we show that FAT10 directly binds to and impedes the activity of the heterodimeric SUMO E1 activating enzyme AOS1/UBA2 by competing very efficiently with SUMO for activation and thioester formation. Nevertheless, activation of FAT10 by AOS1/UBA2 does not lead to covalent conjugation of FAT10 with substrate proteins which relies on its cognate E1 enzyme UBA6. Hence, we report that one ubiquitin-like modifier (FAT10) inhibits the conjugation and function of another ubiquitin-like modifier (SUMO) by impairing its activation.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-10-11T11:19:02Z</dc:date>
    <dc:creator>Aichem, Annette</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/47200"/>
    <dc:creator>Ryu, Stella</dc:creator>
    <dc:contributor>Melchior, Frauke</dc:contributor>
    <dc:contributor>Gröttrup, Marcus</dc:contributor>
    <dc:creator>Sailer, Carolin</dc:creator>
    <dc:contributor>Stankovic-Valentin, Nicolas</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Stengel, Florian</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-10-11T11:19:02Z</dcterms:available>
    <dcterms:issued>2019-10-01</dcterms:issued>
    <dc:contributor>Sailer, Carolin</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:creator>Melchior, Frauke</dc:creator>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:title>The ubiquitin-like modifier FAT10 interferes with SUMO activation</dcterms:title>
    <dc:creator>Schmidtke, Gunter</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Gröttrup, Marcus</dc:creator>
    <dc:contributor>Ryu, Stella</dc:contributor>
    <dc:creator>Catone, Nicola</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/47200/3/Aichem_2-bk0hoidsar8l4.pdf"/>
    <dc:creator>Stankovic-Valentin, Nicolas</dc:creator>
    <dc:contributor>Schmidtke, Gunter</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Catone, Nicola</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen