Publikation:

Microcystin congener-specific in vitro neurotoxicity

Lade...
Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2008

Autor:innen

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Toxicology Letters. Elsevier. 2008, 180, pp. S103. ISSN 0378-4274. eISSN 1879-3169. Available under: doi: 10.1016/j.toxlet.2008.06.419

Zusammenfassung

Contamination of natural waters by cyanobacterial blooms is a worldwide problem, resulting in serious water pollution and health hazards for humans and livestock. The cyanobacterial micro- cystins (MCs) represent a group of >80 cyclic heptapeptide toxin congeners, known to induce hepato-, nephro-, and potentially neu- rotoxic effects via protein phosphatase (PP)-inhibition. Present evidence suggests that organic anion transporting polypeptides (rodent Oatps/human OATPs) are required for active uptake of MCs into hepatocytes and kidney epithelial cells. Based on the presence of Oatps/OATPs at the blood–brain-barrier (BBB) and blood–cerebrospinal fluid-barrier (BCFB) it was hypothesized that MCs can be transported across the BBB/BCFB and into neurons in an Oatp/OATP-dependent manner and will induce neurotoxic effects. To test this hypothesis, primary murine neurons (Cere- bellar Granule Cells, mCGC) were analyzed for the presence of mCGC Oatps (mRNA level). Subsequently, the uptake, localization and neurotoxic effects of MC-LR, -LW, and -LF were investigated using MC-immunoblotting, confocal microscopy of immunostained neurons, PP-inhibition assay, TNF- ELISA and caspase 3/7 activ- ity assay. RT-PCR demonstrated the presence of six murine Oatps (Oatp1c1, 1a5, 3a1, 1a1, 1b2 and 6d1) in mCGC. MC-LR-specific immunodetection demonstrated a concentration-dependent accu- mulation of this congener and covalent binding to PP-1 and -2A. mCGC PP activity was reduced by 20% following 48 h exposure to ≥300 nM MC-LR, -LW and -LF concurrent with a congener and concentration-dependent up regulation of TNF- expression and caspase 3/7 activity. In conclusion, the data suggest an MC congener-dependent uptake and neurotoxicity in primary mouse CGC.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690FEURSTEIN, Daniel, Andreas FISCHER, Daniel R. DIETRICH, 2008. Microcystin congener-specific in vitro neurotoxicity. In: Toxicology Letters. Elsevier. 2008, 180, pp. S103. ISSN 0378-4274. eISSN 1879-3169. Available under: doi: 10.1016/j.toxlet.2008.06.419
BibTex
@article{Feurstein2008Micro-58585,
  year={2008},
  doi={10.1016/j.toxlet.2008.06.419},
  title={Microcystin congener-specific in vitro neurotoxicity},
  volume={180},
  issn={0378-4274},
  journal={Toxicology Letters},
  author={Feurstein, Daniel and Fischer, Andreas and Dietrich, Daniel R.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/58585">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-09T10:38:24Z</dcterms:available>
    <dc:creator>Fischer, Andreas</dc:creator>
    <dc:contributor>Dietrich, Daniel R.</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2008</dcterms:issued>
    <dcterms:title>Microcystin congener-specific in vitro neurotoxicity</dcterms:title>
    <dc:creator>Feurstein, Daniel</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-09-09T10:38:24Z</dc:date>
    <dc:contributor>Feurstein, Daniel</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/58585"/>
    <dc:contributor>Fischer, Andreas</dc:contributor>
    <dc:creator>Dietrich, Daniel R.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">Contamination of natural waters by cyanobacterial blooms is a worldwide problem, resulting in serious water pollution and health hazards for humans and livestock. The cyanobacterial micro- cystins (MCs) represent a group of &gt;80 cyclic heptapeptide toxin congeners, known to induce hepato-, nephro-, and potentially neu- rotoxic effects via protein phosphatase (PP)-inhibition. Present evidence suggests that organic anion transporting polypeptides (rodent Oatps/human OATPs) are required for active uptake of MCs into hepatocytes and kidney epithelial cells. Based on the presence of Oatps/OATPs at the blood–brain-barrier (BBB) and blood–cerebrospinal fluid-barrier (BCFB) it was hypothesized that MCs can be transported across the BBB/BCFB and into neurons in an Oatp/OATP-dependent manner and will induce neurotoxic effects. To test this hypothesis, primary murine neurons (Cere- bellar Granule Cells, mCGC) were analyzed for the presence of mCGC Oatps (mRNA level). Subsequently, the uptake, localization and neurotoxic effects of MC-LR, -LW, and -LF were investigated using MC-immunoblotting, confocal microscopy of immunostained neurons, PP-inhibition assay, TNF-  ELISA and caspase 3/7 activ- ity assay. RT-PCR demonstrated the presence of six murine Oatps (Oatp1c1, 1a5, 3a1, 1a1, 1b2 and 6d1) in mCGC. MC-LR-specific immunodetection demonstrated a concentration-dependent accu- mulation of this congener and covalent binding to PP-1 and -2A. mCGC PP activity was reduced by 20% following 48 h exposure to ≥300 nM MC-LR, -LW and -LF concurrent with a congener and concentration-dependent up regulation of TNF-  expression and caspase 3/7 activity. In conclusion, the data suggest an MC congener-dependent uptake and neurotoxicity in primary mouse CGC.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen