Reflective multi-immersion microscope objectives

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2023
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Voigt, Fabian F.
Naert, Thomas
Lienkamp, Soeren S.
Helmchen, Fritjof
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BROWN, Thomas G., ed., Tony WILSON, ed., Laura WALLER, ed.. Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. Bellingham, Washington: SPIE, 2023, 123850B. Available under: doi: 10.1117/12.2648095
Zusammenfassung

We present a novel way of designing multi-immersion microscope objectives inspired by both the mirror-based eyes of scallops and the Schmidt telescope, a wide-field telescope invented in the 1930s. Despite containing only two optical elements and without a correction collar, our design achieves diffraction-limited imaging performance over mm-scale field-of-views in air and in any homogeneous liquid medium. Based on our concept, we built a prototype two-photon microscope objective and demonstrated its versatility by imaging Xenopus tadpoles cleared with BABB (n=1.56) and by imaging the larval Zebrafish brain in vivo in water.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Objectives, Design and modelling, Microscopes, Mirrors, Water, Liquids, Point spread functions
Konferenz
SPIE BiOS 2023 on Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX, 28. Jan. 2023 - 3. Feb. 2023, San Francisco, CA
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Zitieren
ISO 690VOIGT, Fabian F., Thomas NAERT, Armin BAHL, Soeren S. LIENKAMP, Fritjof HELMCHEN, 2023. Reflective multi-immersion microscope objectives. SPIE BiOS 2023 on Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. San Francisco, CA, 28. Jan. 2023 - 3. Feb. 2023. In: BROWN, Thomas G., ed., Tony WILSON, ed., Laura WALLER, ed.. Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. Bellingham, Washington: SPIE, 2023, 123850B. Available under: doi: 10.1117/12.2648095
BibTex
@inproceedings{Voigt2023Refle-67639,
  year={2023},
  doi={10.1117/12.2648095},
  title={Reflective multi-immersion microscope objectives},
  publisher={SPIE},
  address={Bellingham, Washington},
  booktitle={Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX},
  editor={Brown, Thomas G. and Wilson, Tony and Waller, Laura},
  author={Voigt, Fabian F. and Naert, Thomas and Bahl, Armin and Lienkamp, Soeren S. and Helmchen, Fritjof},
  note={Article Number: 123850B}
}
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