Publikation: Reflective multi-immersion microscope objectives
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2023
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BROWN, Thomas G., ed., Tony WILSON, ed., Laura WALLER, ed.. Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. Bellingham, Washington: SPIE, 2023, 123850B. Available under: doi: 10.1117/12.2648095
Zusammenfassung
We present a novel way of designing multi-immersion microscope objectives inspired by both the mirror-based eyes of scallops and the Schmidt telescope, a wide-field telescope invented in the 1930s. Despite containing only two optical elements and without a correction collar, our design achieves diffraction-limited imaging performance over mm-scale field-of-views in air and in any homogeneous liquid medium. Based on our concept, we built a prototype two-photon microscope objective and demonstrated its versatility by imaging Xenopus tadpoles cleared with BABB (n=1.56) and by imaging the larval Zebrafish brain in vivo in water.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Objectives, Design and modelling, Microscopes, Mirrors, Water, Liquids, Point spread functions
Konferenz
SPIE BiOS 2023 on Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX, 28. Jan. 2023 - 3. Feb. 2023, San Francisco, CA
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VOIGT, Fabian F., Thomas NAERT, Armin BAHL, Soeren S. LIENKAMP, Fritjof HELMCHEN, 2023. Reflective multi-immersion microscope objectives. SPIE BiOS 2023 on Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. San Francisco, CA, 28. Jan. 2023 - 3. Feb. 2023. In: BROWN, Thomas G., ed., Tony WILSON, ed., Laura WALLER, ed.. Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. Bellingham, Washington: SPIE, 2023, 123850B. Available under: doi: 10.1117/12.2648095BibTex
@inproceedings{Voigt2023Refle-67639,
year={2023},
doi={10.1117/12.2648095},
title={Reflective multi-immersion microscope objectives},
publisher={SPIE},
address={Bellingham, Washington},
booktitle={Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX},
editor={Brown, Thomas G. and Wilson, Tony and Waller, Laura},
author={Voigt, Fabian F. and Naert, Thomas and Bahl, Armin and Lienkamp, Soeren S. and Helmchen, Fritjof},
note={Article Number: 123850B}
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<dcterms:abstract>We present a novel way of designing multi-immersion microscope objectives inspired by both the mirror-based eyes of scallops and the Schmidt telescope, a wide-field telescope invented in the 1930s. Despite containing only two optical elements and without a correction collar, our design achieves diffraction-limited imaging performance over mm-scale field-of-views in air and in any homogeneous liquid medium. Based on our concept, we built a prototype two-photon microscope objective and demonstrated its versatility by imaging Xenopus tadpoles cleared with BABB (n=1.56) and by imaging the larval Zebrafish brain in vivo in water.</dcterms:abstract>
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