Reflective multi-immersion microscope objectives

Voigt, Fabian F.; Naert, Thomas; Bahl, Armin; Lienkamp, Soeren S.; Helmchen, Fritjof

Reflective multi-immersion microscope objectives

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2023

Publikationstyp

Beitrag zu einem Konferenzband

Publikationsstatus

Published

Erschienen in

BROWN, Thomas G., ed., Tony WILSON, ed., Laura WALLER, ed.. Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. Bellingham, Washington: SPIE, 2023, 123850B. Available under: doi: 10.1117/12.2648095

Zusammenfassung

We present a novel way of designing multi-immersion microscope objectives inspired by both the mirror-based eyes of scallops and the Schmidt telescope, a wide-field telescope invented in the 1930s. Despite containing only two optical elements and without a correction collar, our design achieves diffraction-limited imaging performance over mm-scale field-of-views in air and in any homogeneous liquid medium. Based on our concept, we built a prototype two-photon microscope objective and demonstrated its versatility by imaging Xenopus tadpoles cleared with BABB (n=1.56) and by imaging the larval Zebrafish brain in vivo in water.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Objectives, Design and modelling, Microscopes, Mirrors, Water, Liquids, Point spread functions

Konferenz

SPIE BiOS 2023 on Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX, 28. Jan. 2023 - 3. Feb. 2023, San Francisco, CA

Zitieren

ISO 690

VOIGT, Fabian F., Thomas NAERT, Armin BAHL, Soeren S. LIENKAMP, Fritjof HELMCHEN, 2023. Reflective multi-immersion microscope objectives. SPIE BiOS 2023 on Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. San Francisco, CA, 28. Jan. 2023 - 3. Feb. 2023. In: BROWN, Thomas G., ed., Tony WILSON, ed., Laura WALLER, ed.. Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX. Bellingham, Washington: SPIE, 2023, 123850B. Available under: doi: 10.1117/12.2648095

BibTex

@inproceedings{Voigt2023Refle-67639,
  year={2023},
  doi={10.1117/12.2648095},
  title={Reflective multi-immersion microscope objectives},
  publisher={SPIE},
  address={Bellingham, Washington},
  booktitle={Proceedings Volume 12385 : Three-Dimensional and Multidimensional Microscopy : Image Acquisition and Processing XXX},
  editor={Brown, Thomas G. and Wilson, Tony and Waller, Laura},
  author={Voigt, Fabian F. and Naert, Thomas and Bahl, Armin and Lienkamp, Soeren S. and Helmchen, Fritjof},
  note={Article Number: 123850B}
}

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