Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation

Lade...
Vorschaubild
Dateien
Sako_2-cb0g7uvvaug75.pdf
Sako_2-cb0g7uvvaug75.pdfGröße: 3.76 MBDownloads: 96
Datum
2016
Autor:innen
Sako, Keisuke
Pradhan, Saurabh J.
Barone, Vanessa
Inglés-Prieto, Álvaro
Ruprecht, Verena
Galande, Sanjeev
Janovjak, Harald
Heisenberg, Carl-Philipp
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Gold
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690SAKO, Keisuke, Saurabh J. PRADHAN, Vanessa BARONE, Álvaro INGLÉS-PRIETO, Patrick MÜLLER, Verena RUPRECHT, Daniel CAPEK, Sanjeev GALANDE, Harald JANOVJAK, Carl-Philipp HEISENBERG, 2016. Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation. In: Cell Reports. Cell Press. 2016, 16(3), pp. 866-877. eISSN 2211-1247. Available under: doi: 10.1016/j.celrep.2016.06.036
BibTex
@article{Sako2016Optog-56572,
  year={2016},
  doi={10.1016/j.celrep.2016.06.036},
  title={Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation},
  number={3},
  volume={16},
  journal={Cell Reports},
  pages={866--877},
  author={Sako, Keisuke and Pradhan, Saurabh J. and Barone, Vanessa and Inglés-Prieto, Álvaro and Müller, Patrick and Ruprecht, Verena and Capek, Daniel and Galande, Sanjeev and Janovjak, Harald and Heisenberg, Carl-Philipp}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/56572">
    <dc:creator>Janovjak, Harald</dc:creator>
    <dc:contributor>Heisenberg, Carl-Philipp</dc:contributor>
    <dc:contributor>Janovjak, Harald</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Sako, Keisuke</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56572/3/Sako_2-cb0g7uvvaug75.pdf"/>
    <dc:creator>Sako, Keisuke</dc:creator>
    <dc:contributor>Barone, Vanessa</dc:contributor>
    <dcterms:title>Optogenetic Control of Nodal Signaling Reveals a Temporal Pattern of Nodal Signaling Regulating Cell Fate Specification during Gastrulation</dcterms:title>
    <dc:contributor>Müller, Patrick</dc:contributor>
    <dc:contributor>Capek, Daniel</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-02-15T15:00:54Z</dcterms:available>
    <dc:creator>Ruprecht, Verena</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2022-02-15T15:00:54Z</dc:date>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/56572"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Galande, Sanjeev</dc:contributor>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/56572/3/Sako_2-cb0g7uvvaug75.pdf"/>
    <dc:creator>Inglés-Prieto, Álvaro</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Capek, Daniel</dc:creator>
    <dc:creator>Müller, Patrick</dc:creator>
    <dc:creator>Heisenberg, Carl-Philipp</dc:creator>
    <dc:contributor>Pradhan, Saurabh J.</dc:contributor>
    <dcterms:issued>2016</dcterms:issued>
    <dc:creator>Barone, Vanessa</dc:creator>
    <dc:contributor>Ruprecht, Verena</dc:contributor>
    <dc:creator>Pradhan, Saurabh J.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.</dcterms:abstract>
    <dc:creator>Galande, Sanjeev</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:language>eng</dc:language>
    <dc:contributor>Inglés-Prieto, Álvaro</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Ja
Diese Publikation teilen