Reversible coupling of individual phycobiliprotein isoforms during state transitions in the cyanobacterium Trichodesmium analysed by single-cell fluorescence kinetic measurements

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2009
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Wiegert, Susanna
imek, Miloslav
etlík, Ivan
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Biochimica et Biophysica Acta / Bioenergetics ; 1787 (2009), 3. - pp. 155-167. - ISSN 0005-2728
Abstract
In the non-heterocyst. marine cyanobacterium Trichodesmium nitrogen fixation is con fined to the photoperiod and occurs coevally with oxygenic photosynthesis although nitrogenase is irreversibly inactivated by oxygen. In previous studies it was found that regulation of photosynthesis for nitrogen fixation involves Mehler reaction and various activity states with reversible coupling of photosynthetic components. We now investigated these activity states in more detail. Spectrally resolved fluorescence kinetic measurements of single cells revealed that they were related to alternate uncoupling and coupling of phycobilisomes from and to the photosystems. changing the effective cross-section of PSI!. Therefore, we isolated and purified the phycobiliproteins of Trichodesmium via ion exchange chromatography and recorded their UV/VIS absorption. fluorescence excitation and fluorescence emission spectra. After describing these spectra by mathematical equations via the Gauss-Peak-Spectra method. we used them to deconvolute the in vivo fluorescence spectra of richodesmium cells. This revealed that the contribution of different parts of the phycobilisome antenna to fluorescence quenching changed during the daily activity cycle. and that individual phycobiliproteins can be reversibly coupled to the photosystems, while the expression levels of these proteins did not change much during the daily activity cycle. Thus we propose that variable phycobilisome coupling plays a key role in the regulation of photosynthesis for nitrogen fixation in Trichodesmium.
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570 Biosciences, Biology
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Fluorescence kinetic microscopy,In vivo spectroscopy,Nitrogen fixation,Photosynthesis,Phycobilisome,State transition
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ISO 690KÜPPER, Hendrik, Elisa ANDRESEN, Susanna WIEGERT, Miloslav IMEK, Barbara LEITENMAIER, Ivan ETLÍK, 2009. Reversible coupling of individual phycobiliprotein isoforms during state transitions in the cyanobacterium Trichodesmium analysed by single-cell fluorescence kinetic measurements. In: Biochimica et Biophysica Acta / Bioenergetics. 1787(3), pp. 155-167. ISSN 0005-2728. Available under: doi: 10.1016/j.bbabio.2009.01.001
BibTex
@article{Kupper2009Rever-8065,
  year={2009},
  doi={10.1016/j.bbabio.2009.01.001},
  title={Reversible coupling of individual phycobiliprotein isoforms during state transitions in the cyanobacterium Trichodesmium analysed by single-cell fluorescence kinetic measurements},
  number={3},
  volume={1787},
  issn={0005-2728},
  journal={Biochimica et Biophysica Acta / Bioenergetics},
  pages={155--167},
  author={Küpper, Hendrik and Andresen, Elisa and Wiegert, Susanna and imek, Miloslav and Leitenmaier, Barbara and etlík, Ivan}
}
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