Complete Ion-Coordination Structure in the Rotor Ring of Na+ -Dependent F-ATP Synthases

Lade...
Vorschaubild
Dateien
Complete_Ion_Coordination.pdf
Complete_Ion_Coordination.pdfGröße: 8.7 MBDownloads: 905
Datum
2009
Autor:innen
Meier, Thomas
Krah, Alexander
Bond, Peter J.
Pogoryelov, Denys
Faraldo-Gómez, José D.
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Journal of Molecular Biology. 2009, 391(2), pp. 498-507. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/j.jmb.2009.05.082
Zusammenfassung

The membrane-embedded rotors of Na(+)-dependent F-ATP synthases comprise 11 c-subunits that form a ring, with 11 Na(+) binding sites in between adjacent subunits. Following an updated crystallographic analysis of the c-ring from Ilyobacter tartaricus, we report the complete ion-coordination structure of the Na(+) sites. In addition to the four residues previously identified, there exists a fifth ligand, namely, a buried structural water molecule. This water is itself coordinated by Thr67, which, sequence analysis reveals, is the only residue involved in binding that distinguishes Na(+) synthases from H(+)-ATP synthases known to date. Molecular dynamics simulations and free-energy calculations of the c-ring in a lipid membrane lend clear support to the notion that this fifth ligand is a water molecule, and illustrate its influence on the selectivity of the binding sites. Given the evolutionary ascendancy of sodium over proton bioenergetics, this structure uncovers an ancient strategy for selective ion coupling in ATP synthases.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
FiFo-ATP synthase rotor, coring structure, ion coordination and selectivity, sodium-motive force, llyobacter tartaricus
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MEIER, Thomas, Alexander KRAH, Peter J. BOND, Denys POGORYELOV, Kay DIEDERICHS, José D. FARALDO-GÓMEZ, 2009. Complete Ion-Coordination Structure in the Rotor Ring of Na+ -Dependent F-ATP Synthases. In: Journal of Molecular Biology. 2009, 391(2), pp. 498-507. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/j.jmb.2009.05.082
BibTex
@article{Meier2009Compl-7963,
  year={2009},
  doi={10.1016/j.jmb.2009.05.082},
  title={Complete Ion-Coordination Structure in the Rotor Ring of Na+ -Dependent F-ATP Synthases},
  number={2},
  volume={391},
  issn={0022-2836},
  journal={Journal of Molecular Biology},
  pages={498--507},
  author={Meier, Thomas and Krah, Alexander and Bond, Peter J. and Pogoryelov, Denys and Diederichs, Kay and Faraldo-Gómez, José D.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7963">
    <dc:creator>Diederichs, Kay</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7963/1/Complete_Ion_Coordination.pdf"/>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:38:54Z</dc:date>
    <dc:contributor>Bond, Peter J.</dc:contributor>
    <dc:contributor>Faraldo-Gómez, José D.</dc:contributor>
    <dc:creator>Meier, Thomas</dc:creator>
    <dc:creator>Bond, Peter J.</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Faraldo-Gómez, José D.</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7963"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:issued>2009</dcterms:issued>
    <dc:creator>Krah, Alexander</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Pogoryelov, Denys</dc:contributor>
    <dc:creator>Pogoryelov, Denys</dc:creator>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7963/1/Complete_Ion_Coordination.pdf"/>
    <dcterms:abstract xml:lang="eng">The membrane-embedded rotors of Na(+)-dependent F-ATP synthases comprise 11 c-subunits that form a ring, with 11 Na(+) binding sites in between adjacent subunits. Following an updated crystallographic analysis of the c-ring from Ilyobacter tartaricus, we report the complete ion-coordination structure of the Na(+) sites. In addition to the four residues previously identified, there exists a fifth ligand, namely, a buried structural water molecule. This water is itself coordinated by Thr67, which, sequence analysis reveals, is the only residue involved in binding that distinguishes Na(+) synthases from H(+)-ATP synthases known to date. Molecular dynamics simulations and free-energy calculations of the c-ring in a lipid membrane lend clear support to the notion that this fifth ligand is a water molecule, and illustrate its influence on the selectivity of the binding sites. Given the evolutionary ascendancy of sodium over proton bioenergetics, this structure uncovers an ancient strategy for selective ion coupling in ATP synthases.</dcterms:abstract>
    <dcterms:bibliographicCitation>First publ. in: Journal of Molecular Biology ; 391 (2009), 2. - S. 498-507</dcterms:bibliographicCitation>
    <dc:contributor>Meier, Thomas</dc:contributor>
    <dc:contributor>Krah, Alexander</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dcterms:title>Complete Ion-Coordination Structure in the Rotor Ring of Na+ -Dependent F-ATP Synthases</dcterms:title>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen