Interaction of beta-amyloid(1-40) peptide with pairs of metal ions : an electrospray ion trap mass spectrometric model study

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2009
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Drochioiu, Gabi
Drăguşanu, Mihaela
Murariu, Manuela
Dragan, Ecaterina Stela
Petre, Brînduşa-Alina
Mezö, Gábor
Przybylski, Michael
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The stoichiometries and the affinity toward simple and paired metal ions of synthetic amyloid-β(1-40) peptide (Aβ1-40) were investigated by electrospray ion trap mass spectrometry (ESI-MS), circular dichroism (CD), and atomic force microscopy (AFM). The results lead to the working hypothesis that pH-dependent metal binding to Aβ1-40 may induce conformational changes, which affect the affinity toward other metals. A significant copper and zinc binding to Aβ1-40 peptide at pH 5.5 was found, whereas nickel ions commonly bind to each molecule of β-amyloid peptide. Some complexes of Aβ1-40 with more than one nickel ion were identified by ESI-MS. In addition, nickel ions proved to enhance Aβ oligomerization. On increasing pH, up to 12 ions of zinc may bind to a single Aβ molecule. Under the same pH and concentration conditions, the binding pattern of the independent copper and silver ions to Aβ1-40 was different from that of the equimolecular mixture of the two metal ions. One might assume that some conformational changes due to water loss altered the capacity of Aβ peptide to bind certain heavy metal ions. As a consequence, copper silver interaction with the binding process to Aβ1-40 became highly complex. A competition between silver and nickel ions for Aβ1-40 binding sites at high pH was also observed. New strategies were proposed to identify the characteristic signals for some important metal ion peptide complexes in the spectra recorded at high pH or high concentrations of metal ions. To explain the formation of such a large number of high metal ion Aβ complexes, we took into consideration the participation of both histidine residues and free amino groups as well as carboxylate ones in the binding process. Finally, CD and AFM studies supported the mass spectrometric data.

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540 Chemie
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Amyloid beta-peptide, Metal ion peptide complex, Alzheimer's disease, ESI ion trap MS
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ISO 690DROCHIOIU, Gabi, Marilena MANEA, Mihaela DRĂGUŞANU, Manuela MURARIU, Ecaterina Stela DRAGAN, Brînduşa-Alina PETRE, Gábor MEZÖ, Michael PRZYBYLSKI, 2009. Interaction of beta-amyloid(1-40) peptide with pairs of metal ions : an electrospray ion trap mass spectrometric model study. In: Biophysical Chemistry. 2009, 144(1-2), pp. 9-20. ISSN 0301-4622. eISSN 1873-4200. Available under: doi: 10.1016/j.bpc.2009.05.008
BibTex
@article{Drochioiu2009Inter-9690,
  year={2009},
  doi={10.1016/j.bpc.2009.05.008},
  title={Interaction of beta-amyloid(1-40) peptide with pairs of metal ions : an electrospray ion trap mass spectrometric model study},
  number={1-2},
  volume={144},
  issn={0301-4622},
  journal={Biophysical Chemistry},
  pages={9--20},
  author={Drochioiu, Gabi and Manea, Marilena and Drăguşanu, Mihaela and Murariu, Manuela and Dragan, Ecaterina Stela and Petre, Brînduşa-Alina and Mezö, Gábor and Przybylski, Michael}
}
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    <dcterms:abstract xml:lang="eng">The stoichiometries and the affinity toward simple and paired metal ions of synthetic amyloid-β(1-40) peptide (Aβ1-40) were investigated by electrospray ion trap mass spectrometry (ESI-MS), circular dichroism (CD), and atomic force microscopy (AFM). The results lead to the working hypothesis that pH-dependent metal binding to Aβ1-40 may induce conformational changes, which affect the affinity toward other metals. A significant copper and zinc binding to Aβ1-40 peptide at pH 5.5 was found, whereas nickel ions commonly bind to each molecule of β-amyloid peptide. Some complexes of Aβ1-40 with more than one nickel ion were identified by ESI-MS. In addition, nickel ions proved to enhance Aβ oligomerization. On increasing pH, up to 12 ions of zinc may bind to a single Aβ molecule. Under the same pH and concentration conditions, the binding pattern of the independent copper and silver ions to Aβ1-40 was different from that of the equimolecular mixture of the two metal ions. One might assume that some conformational changes due to water loss altered the capacity of Aβ peptide to bind certain heavy metal ions. As a consequence, copper silver interaction with the binding process to Aβ1-40 became highly complex. A competition between silver and nickel ions for Aβ1-40 binding sites at high pH was also observed. New strategies were proposed to identify the characteristic signals for some important metal ion peptide complexes in the spectra recorded at high pH or high concentrations of metal ions. To explain the formation of such a large number of high metal ion Aβ complexes, we took into consideration the participation of both histidine residues and free amino groups as well as carboxylate ones in the binding process. Finally, CD and AFM studies supported the mass spectrometric data.</dcterms:abstract>
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