Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopes

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2008
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Jakob, Sabine
Corazza, Nadia
Diamantis, Eva
Kappeler, Andreas
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Methods. 2008, 44(3), pp. 255-261. ISSN 1046-2023. Available under: doi: 10.1016/j.ymeth.2007.11.004
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Cell death induction by apoptosis is an important process in the maintenance of tissue homeostasis as well as tissue destruction during various pathological processes. Consequently, detection of apoptotic cells in situ represents an important technique to assess the extent and impact of cell death in the respective tissue. While scoring of apoptosis by histological assessment of apoptotic cells is still a widely used method, it is likely biased by sensitivity problems and observed-based variations. The availability of caspase-mediated neo-epitope-specific antibodies offers new tools for the detection of apoptosis in situ. Here, we discuss the use of immunohistochemical detection of cleaved caspase 3 and lamin A for the assessment of apoptotic cells in paraffin-embedded liver tissue. Furthermore, we evaluate the effect of tissue pretreatment and antigen retrieval on the sensitivity of apoptosis detection, background staining and maintenance of tissue morphology.

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570 Biowissenschaften, Biologie
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ISO 690JAKOB, Sabine, Nadia CORAZZA, Eva DIAMANTIS, Andreas KAPPELER, Thomas BRUNNER, 2008. Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopes. In: Methods. 2008, 44(3), pp. 255-261. ISSN 1046-2023. Available under: doi: 10.1016/j.ymeth.2007.11.004
BibTex
@article{Jakob2008-03Detec-14269,
  year={2008},
  doi={10.1016/j.ymeth.2007.11.004},
  title={Detection of apoptosis in vivo using antibodies against caspase-induced neo-epitopes},
  number={3},
  volume={44},
  issn={1046-2023},
  journal={Methods},
  pages={255--261},
  author={Jakob, Sabine and Corazza, Nadia and Diamantis, Eva and Kappeler, Andreas and Brunner, Thomas}
}
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