Publikation: Methods to Study Signaling at the Golgi Apparatus
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2013
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PEREZ, Franck, ed. and others. Methods for analysis of Golgi complex function. 1. ed.. Amsterdam: Elsevier, 2013, pp. 345-358. Methods in cell biology. 118. ISBN 978-0-12-417164-0. Available under: doi: 10.1016/B978-0-12-417164-0.00021-5
Zusammenfassung
Research on the secretory pathway in the past three decades accounts for our known knowledge about the composition and architecture of organelles and about the machinery that regulates membrane transport. An emerging topic in the past few years was the discovery that the secretory pathway is regulated by signaling, and in this regard, the Golgi apparatus received major attention. In the current chapter, we will highlight various techniques that are used by us and others to study signaling at the Golgi. We describe methods to study lipid and protein phosphorylation at the Golgi and various techniques for studying spatial activation of GTPases at this organelle. We also discuss how combining these techniques and improving their limitations is important for gaining a better understanding of how the Golgi intersects with various signal transduction pathways.
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570 Biowissenschaften, Biologie
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REITERE, Veronika, Francesco BASCHIERI, Valentina MILLARTE, Hesso FARHAN, 2013. Methods to Study Signaling at the Golgi Apparatus. In: PEREZ, Franck, ed. and others. Methods for analysis of Golgi complex function. 1. ed.. Amsterdam: Elsevier, 2013, pp. 345-358. Methods in cell biology. 118. ISBN 978-0-12-417164-0. Available under: doi: 10.1016/B978-0-12-417164-0.00021-5BibTex
@incollection{Reitere2013Metho-42006,
year={2013},
doi={10.1016/B978-0-12-417164-0.00021-5},
title={Methods to Study Signaling at the Golgi Apparatus},
edition={1. ed.},
number={118},
isbn={978-0-12-417164-0},
publisher={Elsevier},
address={Amsterdam},
series={Methods in cell biology},
booktitle={Methods for analysis of Golgi complex function},
pages={345--358},
editor={Perez, Franck},
author={Reitere, Veronika and Baschieri, Francesco and Millarte, Valentina and Farhan, Hesso}
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<dcterms:abstract xml:lang="eng">Research on the secretory pathway in the past three decades accounts for our known knowledge about the composition and architecture of organelles and about the machinery that regulates membrane transport. An emerging topic in the past few years was the discovery that the secretory pathway is regulated by signaling, and in this regard, the Golgi apparatus received major attention. In the current chapter, we will highlight various techniques that are used by us and others to study signaling at the Golgi. We describe methods to study lipid and protein phosphorylation at the Golgi and various techniques for studying spatial activation of GTPases at this organelle. We also discuss how combining these techniques and improving their limitations is important for gaining a better understanding of how the Golgi intersects with various signal transduction pathways.</dcterms:abstract>
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