Publikation: Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Chronic stress is associated with a higher risk for carcinogenesis as well as age-related diseases and immune dysfunction. There is evidence showing that psychological stress can contribute to premature immunosenescence. Therefore, the question arose whether chronic exposure to catecholamine could drive immune cells into senescence. Peripheral blood mononuclear cells were isolated from whole blood. After repeated ex vivo treatment with isoproterenol, an epinephrine analog, well-established senescence biomarkers were assessed. We found (i) DNA double-strand break induction, (ii) telomere shortening, (iii) failure to proliferate, (iv) higher senescence-associated β-galactosidase activity, (v) decreases in caspases 3 and 7 activity, and (vi) strong upregulation of the proteoglycan versican accompanied by increased cellular adhesion suggesting the induction of a senescence-like phenotype. These results emphasize the complexity of the effect of isoproterenol on multiple cellular processes and provide insights into the molecular mechanisms of stress leading to immunosenescence.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
LAUMANN, Michael, Philipp PALOMBO, Judy FIERES, Mara THOMAS, Gabriele SARETZKI, Alexander BÜRKLE, Maria MORENO-VILLANUEVA, 2024. Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells. In: Biomolecules. MDPI. 2024, 14(12), 1528. eISSN 2218-273X. Verfügbar unter: doi: 10.3390/biom14121528BibTex
@article{Laumann2024-11-28Senes-71512, year={2024}, doi={10.3390/biom14121528}, title={Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells}, number={12}, volume={14}, journal={Biomolecules}, author={Laumann, Michael and Palombo, Philipp and Fieres, Judy and Thomas, Mara and Saretzki, Gabriele and Bürkle, Alexander and Moreno-Villanueva, Maria}, note={Article Number: 1528} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/71512"> <dc:language>eng</dc:language> <dc:contributor>Fieres, Judy</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:rights>Attribution 4.0 International</dc:rights> <dcterms:issued>2024-11-28</dcterms:issued> <dc:creator>Saretzki, Gabriele</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/71512/1/Laumann_2-e1atd7jri36g5.pdf"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Fieres, Judy</dc:creator> <dcterms:abstract>Chronic stress is associated with a higher risk for carcinogenesis as well as age-related diseases and immune dysfunction. There is evidence showing that psychological stress can contribute to premature immunosenescence. Therefore, the question arose whether chronic exposure to catecholamine could drive immune cells into senescence. Peripheral blood mononuclear cells were isolated from whole blood. After repeated ex vivo treatment with isoproterenol, an epinephrine analog, well-established senescence biomarkers were assessed. We found (i) DNA double-strand break induction, (ii) telomere shortening, (iii) failure to proliferate, (iv) higher senescence-associated β-galactosidase activity, (v) decreases in caspases 3 and 7 activity, and (vi) strong upregulation of the proteoglycan versican accompanied by increased cellular adhesion suggesting the induction of a senescence-like phenotype. These results emphasize the complexity of the effect of isoproterenol on multiple cellular processes and provide insights into the molecular mechanisms of stress leading to immunosenescence.</dcterms:abstract> <dc:creator>Bürkle, Alexander</dc:creator> <dc:creator>Laumann, Michael</dc:creator> <dc:contributor>Moreno-Villanueva, Maria</dc:contributor> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/71512/1/Laumann_2-e1atd7jri36g5.pdf"/> <dc:contributor>Thomas, Mara</dc:contributor> <dc:creator>Palombo, Philipp</dc:creator> <dc:contributor>Bürkle, Alexander</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-12-02T09:06:39Z</dc:date> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dc:contributor>Palombo, Philipp</dc:contributor> <dcterms:title>Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-12-02T09:06:39Z</dcterms:available> <dc:creator>Moreno-Villanueva, Maria</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/71512"/> <dc:contributor>Laumann, Michael</dc:contributor> <dc:creator>Thomas, Mara</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Saretzki, Gabriele</dc:contributor> </rdf:Description> </rdf:RDF>