Publikation:

Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells

Lade...
Vorschaubild

Dateien

Laumann_2-e1atd7jri36g5.pdf
Laumann_2-e1atd7jri36g5.pdfGröße: 2.06 MBDownloads: 14

Datum

2024

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Link zur Lizenz

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Gold
Core Facility der Universität Konstanz
Electron Microscopy Centre

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Biomolecules. MDPI. 2024, 14(12), 1528. eISSN 2218-273X. Verfügbar unter: doi: 10.3390/biom14121528

Zusammenfassung

Chronic stress is associated with a higher risk for carcinogenesis as well as age-related diseases and immune dysfunction. There is evidence showing that psychological stress can contribute to premature immunosenescence. Therefore, the question arose whether chronic exposure to catecholamine could drive immune cells into senescence. Peripheral blood mononuclear cells were isolated from whole blood. After repeated ex vivo treatment with isoproterenol, an epinephrine analog, well-established senescence biomarkers were assessed. We found (i) DNA double-strand break induction, (ii) telomere shortening, (iii) failure to proliferate, (iv) higher senescence-associated β-galactosidase activity, (v) decreases in caspases 3 and 7 activity, and (vi) strong upregulation of the proteoglycan versican accompanied by increased cellular adhesion suggesting the induction of a senescence-like phenotype. These results emphasize the complexity of the effect of isoproterenol on multiple cellular processes and provide insights into the molecular mechanisms of stress leading to immunosenescence.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

DNA strand breaks, catecholamine, isoproterenol, cellular senescence, β-galactosidase activity

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690LAUMANN, Michael, Philipp PALOMBO, Judy FIERES, Mara THOMAS, Gabriele SARETZKI, Alexander BÜRKLE, Maria MORENO-VILLANUEVA, 2024. Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells. In: Biomolecules. MDPI. 2024, 14(12), 1528. eISSN 2218-273X. Verfügbar unter: doi: 10.3390/biom14121528
BibTex
@article{Laumann2024-11-28Senes-71512,
  year={2024},
  doi={10.3390/biom14121528},
  title={Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells},
  number={12},
  volume={14},
  journal={Biomolecules},
  author={Laumann, Michael and Palombo, Philipp and Fieres, Judy and Thomas, Mara and Saretzki, Gabriele and Bürkle, Alexander and Moreno-Villanueva, Maria},
  note={Article Number: 1528}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/71512">
    <dc:language>eng</dc:language>
    <dc:contributor>Fieres, Judy</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dcterms:issued>2024-11-28</dcterms:issued>
    <dc:creator>Saretzki, Gabriele</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/71512/1/Laumann_2-e1atd7jri36g5.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Fieres, Judy</dc:creator>
    <dcterms:abstract>Chronic stress is associated with a higher risk for carcinogenesis as well as age-related diseases and immune dysfunction. There is evidence showing that psychological stress can contribute to premature immunosenescence. Therefore, the question arose whether chronic exposure to catecholamine could drive immune cells into senescence. Peripheral blood mononuclear cells were isolated from whole blood. After repeated ex vivo treatment with isoproterenol, an epinephrine analog, well-established senescence biomarkers were assessed. We found (i) DNA double-strand break induction, (ii) telomere shortening, (iii) failure to proliferate, (iv) higher senescence-associated β-galactosidase activity, (v) decreases in caspases 3 and 7 activity, and (vi) strong upregulation of the proteoglycan versican accompanied by increased cellular adhesion suggesting the induction of a senescence-like phenotype. These results emphasize the complexity of the effect of isoproterenol on multiple cellular processes and provide insights into the molecular mechanisms of stress leading to immunosenescence.</dcterms:abstract>
    <dc:creator>Bürkle, Alexander</dc:creator>
    <dc:creator>Laumann, Michael</dc:creator>
    <dc:contributor>Moreno-Villanueva, Maria</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/71512/1/Laumann_2-e1atd7jri36g5.pdf"/>
    <dc:contributor>Thomas, Mara</dc:contributor>
    <dc:creator>Palombo, Philipp</dc:creator>
    <dc:contributor>Bürkle, Alexander</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-12-02T09:06:39Z</dc:date>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:contributor>Palombo, Philipp</dc:contributor>
    <dcterms:title>Senescence-like Phenotype After Chronic Exposure to Isoproterenol in Primary Quiescent Immune Cells</dcterms:title>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2024-12-02T09:06:39Z</dcterms:available>
    <dc:creator>Moreno-Villanueva, Maria</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/71512"/>
    <dc:contributor>Laumann, Michael</dc:contributor>
    <dc:creator>Thomas, Mara</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Saretzki, Gabriele</dc:contributor>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen