Publikation:

Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum : effect of surfactants and organic solvents on activity

Lade...
Vorschaubild

Datum

1999

Autor:innen

Pollegioni, Loredano
Gadda, Giovanni
Ambrosius, Dorothea
Pilone, Mirella S.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Cholesterol Oxidase
Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Biotechnology and Applied Biochemistry. 1999, 30, pp. 27-33. Available under: doi: 10.1111/j.1470-8744.1999.tb01155.x

Zusammenfassung

We have studied systematically the effect of the non-ionic surfactants Thesit and Triton X-100, and of propan-2-ol (used as a substrate solubilizer) on the activity of the cholesterol oxidases from Streptomyces hygroscopicus (SCO) and Brevibacterium sterolicum (BCO). Low concentrations of Thesit lead to an activity increase with both enzymes; at higher surfactant concentrations the opposite effect occurs. Triton X-100 inactivates both enzymes at all concentrations. It is deduced that these surfactants exert their effects by interaction with the enzymes and not by affecting micellar phenomena. The effect of propan-2-ol on SCO, in contrast with that on BCO, depends on the buffer concentration (potassium phosphate). Other organic solvents induce results similar to those obtained with SCO and propan-2-ol. A significant difference between the two cholesterol oxidases emerges when stability is tested at 25 °C and in the presence of different concentrations of propan-2-ol: BCO activity is rapidly inactivated, whereas SCO still has 70% of the initial activity after 5 h in the presence of 30% propan-2-ol. From our results, SCO seems to be the catalyst of choice in comparison with BCO for the exploitation of cholesterol oxidases in biotechnology and applied biochemistry.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690POLLEGIONI, Loredano, Giovanni GADDA, Dorothea AMBROSIUS, Sandro GHISLA, Mirella S. PILONE, 1999. Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum : effect of surfactants and organic solvents on activity. In: Biotechnology and Applied Biochemistry. 1999, 30, pp. 27-33. Available under: doi: 10.1111/j.1470-8744.1999.tb01155.x
BibTex
@article{Pollegioni1999Chole-8659,
  year={1999},
  doi={10.1111/j.1470-8744.1999.tb01155.x},
  title={Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum : effect of surfactants and organic solvents on activity},
  volume={30},
  journal={Biotechnology and Applied Biochemistry},
  pages={27--33},
  author={Pollegioni, Loredano and Gadda, Giovanni and Ambrosius, Dorothea and Ghisla, Sandro and Pilone, Mirella S.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8659">
    <dc:language>eng</dc:language>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dc:creator>Ambrosius, Dorothea</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>First publ. in: Biotechnology and Applied Biochemistry ; 30 (1999). - S. 27-33</dcterms:bibliographicCitation>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:29Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:29Z</dc:date>
    <dcterms:issued>1999</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8659/1/Cholesterol_oxidase_from_Streptomyces_hygroscopicus_and_Brevibacterium_sterolicum.pdf"/>
    <dc:contributor>Ambrosius, Dorothea</dc:contributor>
    <dcterms:title>Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum : effect of surfactants and organic solvents on activity</dcterms:title>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:contributor>Gadda, Giovanni</dc:contributor>
    <dc:creator>Pilone, Mirella S.</dc:creator>
    <dc:creator>Gadda, Giovanni</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <dc:creator>Pollegioni, Loredano</dc:creator>
    <dc:contributor>Pollegioni, Loredano</dc:contributor>
    <dc:contributor>Pilone, Mirella S.</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:abstract xml:lang="eng">We have studied systematically the effect of the non-ionic surfactants Thesit and Triton X-100, and of propan-2-ol (used as a substrate solubilizer) on the activity of the cholesterol oxidases from Streptomyces hygroscopicus (SCO) and Brevibacterium sterolicum (BCO). Low concentrations of Thesit lead to an activity increase with both enzymes; at higher surfactant concentrations the opposite effect occurs. Triton X-100 inactivates both enzymes at all concentrations. It is deduced that these surfactants exert their effects by interaction with the enzymes and not by affecting micellar phenomena. The effect of propan-2-ol on SCO, in contrast with that on BCO, depends on the buffer concentration (potassium phosphate). Other organic solvents induce results similar to those obtained with SCO and propan-2-ol. A significant difference between the two cholesterol oxidases emerges when stability is tested at 25 °C and in the presence of different concentrations of propan-2-ol: BCO activity is rapidly inactivated, whereas SCO still has 70% of the initial activity after 5 h in the presence of 30% propan-2-ol. From our results, SCO seems to be the catalyst of choice in comparison with BCO for the exploitation of cholesterol oxidases in biotechnology and applied biochemistry.</dcterms:abstract>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8659"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8659/1/Cholesterol_oxidase_from_Streptomyces_hygroscopicus_and_Brevibacterium_sterolicum.pdf"/>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen