Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum : effect of surfactants and organic solvents on activity

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1999
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Pollegioni, Loredano
Gadda, Giovanni
Ambrosius, Dorothea
Pilone, Mirella S.
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Cholesterol Oxidase
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Biotechnology and Applied Biochemistry ; 30 (1999). - S. 27-33
Zusammenfassung
We have studied systematically the effect of the non-ionic surfactants Thesit and Triton X-100, and of propan-2-ol (used as a substrate solubilizer) on the activity of the cholesterol oxidases from Streptomyces hygroscopicus (SCO) and Brevibacterium sterolicum (BCO). Low concentrations of Thesit lead to an activity increase with both enzymes; at higher surfactant concentrations the opposite effect occurs. Triton X-100 inactivates both enzymes at all concentrations. It is deduced that these surfactants exert their effects by interaction with the enzymes and not by affecting micellar phenomena. The effect of propan-2-ol on SCO, in contrast with that on BCO, depends on the buffer concentration (potassium phosphate). Other organic solvents induce results similar to those obtained with SCO and propan-2-ol. A significant difference between the two cholesterol oxidases emerges when stability is tested at 25 °C and in the presence of different concentrations of propan-2-ol: BCO activity is rapidly inactivated, whereas SCO still has 70% of the initial activity after 5 h in the presence of 30% propan-2-ol. From our results, SCO seems to be the catalyst of choice in comparison with BCO for the exploitation of cholesterol oxidases in biotechnology and applied biochemistry.
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570 Biowissenschaften, Biologie
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ISO 690POLLEGIONI, Loredano, Giovanni GADDA, Dorothea AMBROSIUS, Sandro GHISLA, Mirella S. PILONE, 1999. Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum : effect of surfactants and organic solvents on activity. In: Biotechnology and Applied Biochemistry. 30, pp. 27-33. Available under: doi: 10.1111/j.1470-8744.1999.tb01155.x
BibTex
@article{Pollegioni1999Chole-8659,
  year={1999},
  doi={10.1111/j.1470-8744.1999.tb01155.x},
  title={Cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum : effect of surfactants and organic solvents on activity},
  volume={30},
  journal={Biotechnology and Applied Biochemistry},
  pages={27--33},
  author={Pollegioni, Loredano and Gadda, Giovanni and Ambrosius, Dorothea and Ghisla, Sandro and Pilone, Mirella S.}
}
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