Cutting edge : neosynthesis is required for the presentation of a T cell epitope from a long-lived viral protein

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2001
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Khan, Selina
De Giuli, Rita
Bruns, Michael
Buchmeier, Michael
Van den Broek, Maries
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Journal of Immunology. 2001, 167(9), pp. 4801-4804. ISSN 0022-1767. eISSN 1550-6606. Available under: doi: 10.4049/jimmunol.167.9.4801
Zusammenfassung

CTLs recognize peptide epitopes which are proteolytically generated by the proteasome and presented on MHC class I molecules. According to the defective ribosomal product (DRiP) hypothesis, epitopes originate from newly synthesized polypeptides which are degraded shortly after their translation. The DRiP hypothesis would explain how epitopes can be generated from long-lived proteins. We examined whether neosynthesis is required for presentation of the immunodominant epitope NP118 of the lymphocytic choriomeningitis virus nucleoprotein, which has a half-life of >3 days. Two days after nucleoprotein biosynthesis was terminated in a tetracycline-regulated transfectant, the presentation of the NP118 epitope ceased. This indicates that NP118 epitopes are generated from newly synthesized nucleoproteins rather than from the long-lived pool of nucleoproteins in the cell. Therefore, the lymphocytic choriomeningitis virus nucleoprotein is the first substrate for which a major prediction of the DRiP hypothesis, namely the requirement for neosynthesis, is shown to hold true.

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570 Biowissenschaften, Biologie
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ISO 690KHAN, Selina, Rita DE GIULI, Gunter SCHMIDTKE, Michael BRUNS, Michael BUCHMEIER, Maries VAN DEN BROEK, Marcus GRÖTTRUP, 2001. Cutting edge : neosynthesis is required for the presentation of a T cell epitope from a long-lived viral protein. In: Journal of Immunology. 2001, 167(9), pp. 4801-4804. ISSN 0022-1767. eISSN 1550-6606. Available under: doi: 10.4049/jimmunol.167.9.4801
BibTex
@article{Khan2001Cutti-22142,
  year={2001},
  doi={10.4049/jimmunol.167.9.4801},
  title={Cutting edge : neosynthesis is required for the presentation of a T cell epitope from a long-lived viral protein},
  number={9},
  volume={167},
  issn={0022-1767},
  journal={Journal of Immunology},
  pages={4801--4804},
  author={Khan, Selina and De Giuli, Rita and Schmidtke, Gunter and Bruns, Michael and Buchmeier, Michael and Van den Broek, Maries and Gröttrup, Marcus}
}
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