Enzyme activities in and energetics of acetate metabolism by the mesophilic syntrophically acetate-oxidizing anaerobe Clostridium ultunense

Schnürer, Anna; Svensson, Bo H.; Schink, Bernhard

Enzyme activities in and energetics of acetate metabolism by the mesophilic syntrophically acetate-oxidizing anaerobe Clostridium ultunense

Files

Sammelmappe1.pdf(5.17 MB)

Date

1997

Authors

Schnürer, Anna

Svensson, Bo H.

Schink, Bernhard

Publication type

Journal article

Published in

FEMS Microbiology Letters ; 154 (1997), 2. - pp. 331-336. - eISSN 0378-1097

Abstract

The metabolism of the syntrophically acetate-oxidizing was investigated with cell extracts of the pure culture and the methanogenic triculture from which C. ultunense was isolated. Enzyme measurements indicated that: (1) the CO dehydrogenase (Wood) pathway was used both during acetate formation and during acetate oxidation; (2) methylene-tetrahydrofolate reductase activity was not detected during acetate oxidation; (3) two different methylene-tetrahydrofolate dehydrogenase enzymes were active, depending on the direction in which the Wood pathway was used. The hydrogen partial pressure in the headspace of a growing triculture varied between 1.6 and 6.8 Pa, indicating that C. ultunense and the methanogenic partner each get about 317 kJ per mol reaction in this syntrophic cooperation, thus operating at the lowermost range of energy to be exploited by a living organism.

Subject (DDC)

570 Biosciences, Biology

Keywords

Syntrophic acetate oxidatiion,Clostridium ultunense,enzyme activity,hydrogen partial pressure,energetics

Cite This

ISO 690

SCHNÜRER, Anna, Bo H. SVENSSON, Bernhard SCHINK, 1997. Enzyme activities in and energetics of acetate metabolism by the mesophilic syntrophically acetate-oxidizing anaerobe Clostridium ultunense. In: FEMS Microbiology Letters. 154(2), pp. 331-336. eISSN 0378-1097. Available under: doi: 10.1111/j.1574-6968.1997.tb12664.x

BibTex

@article{Schnurer1997Enzym-7412,
  year={1997},
  doi={10.1111/j.1574-6968.1997.tb12664.x},
  title={Enzyme activities in and energetics of acetate metabolism by the mesophilic syntrophically acetate-oxidizing anaerobe Clostridium ultunense},
  number={2},
  volume={154},
  journal={FEMS Microbiology Letters},
  pages={331--336},
  author={Schnürer, Anna and Svensson, Bo H. and Schink, Bernhard}
}

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