Publikation: Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin
Lade...
Dateien
Datum
2016
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Bioorganic & Medicinal Chemistry. 2016, 24(5), pp. 995-1001. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2016.01.027
Zusammenfassung
The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) has become increasingly important in the conjugation chemistry of biomolecules. For example, it is an efficient and convenient method to generate defined ubiquitin-protein conjugates. Here, we investigate the effect of surfactants on the efficiency of CuAAC for chemical protein ubiquitylation. We found that anionic surfactants enhance conjugate formation by up to 10-fold resulting in high yields even at low (i.e., micromolar) concentrations of the reactants. Notably, the herein investigated conjugates are functional and thus properly folded.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690
SCHNEIDER, Daniel, Tatjana SCHNEIDER, Joos ASCHENBRENNER, Franziska MORTENSEN, Martin SCHEFFNER, Andreas MARX, 2016. Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin. In: Bioorganic & Medicinal Chemistry. 2016, 24(5), pp. 995-1001. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2016.01.027BibTex
@article{Schneider2016-03Anion-33623,
year={2016},
doi={10.1016/j.bmc.2016.01.027},
title={Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin},
number={5},
volume={24},
issn={0968-0896},
journal={Bioorganic & Medicinal Chemistry},
pages={995--1001},
author={Schneider, Daniel and Schneider, Tatjana and Aschenbrenner, Joos and Mortensen, Franziska and Scheffner, Martin and Marx, Andreas}
}RDF
<rdf:RDF
xmlns:dcterms="http://purl.org/dc/terms/"
xmlns:dc="http://purl.org/dc/elements/1.1/"
xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
xmlns:bibo="http://purl.org/ontology/bibo/"
xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
xmlns:foaf="http://xmlns.com/foaf/0.1/"
xmlns:void="http://rdfs.org/ns/void#"
xmlns:xsd="http://www.w3.org/2001/XMLSchema#" >
<rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/33623">
<dc:creator>Schneider, Tatjana</dc:creator>
<dc:contributor>Aschenbrenner, Joos</dc:contributor>
<bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/33623"/>
<dc:contributor>Mortensen, Franziska</dc:contributor>
<dc:contributor>Scheffner, Martin</dc:contributor>
<dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-04-20T07:04:23Z</dcterms:available>
<dcterms:title>Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin</dcterms:title>
<dc:language>eng</dc:language>
<dc:contributor>Marx, Andreas</dc:contributor>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33623/1/Schneider_0-325687.pdf"/>
<dc:creator>Schneider, Daniel</dc:creator>
<dc:creator>Marx, Andreas</dc:creator>
<dcterms:abstract xml:lang="eng">The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) has become increasingly important in the conjugation chemistry of biomolecules. For example, it is an efficient and convenient method to generate defined ubiquitin-protein conjugates. Here, we investigate the effect of surfactants on the efficiency of CuAAC for chemical protein ubiquitylation. We found that anionic surfactants enhance conjugate formation by up to 10-fold resulting in high yields even at low (i.e., micromolar) concentrations of the reactants. Notably, the herein investigated conjugates are functional and thus properly folded.</dcterms:abstract>
<dc:creator>Scheffner, Martin</dc:creator>
<void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:contributor>Schneider, Tatjana</dc:contributor>
<dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
<foaf:homepage rdf:resource="http://localhost:8080/"/>
<dc:creator>Aschenbrenner, Joos</dc:creator>
<dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
<dc:contributor>Schneider, Daniel</dc:contributor>
<dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
<dc:rights>terms-of-use</dc:rights>
<dcterms:issued>2016-03</dcterms:issued>
<dc:creator>Mortensen, Franziska</dc:creator>
<dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-04-20T07:04:23Z</dc:date>
<dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/33623/1/Schneider_0-325687.pdf"/>
</rdf:Description>
</rdf:RDF>Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
