Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin
Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin
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2016
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Bioorganic & Medicinal Chemistry ; 24 (2016), 5. - pp. 995-1001. - ISSN 0968-0896. - eISSN 1464-3391
Abstract
The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) has become increasingly important in the conjugation chemistry of biomolecules. For example, it is an efficient and convenient method to generate defined ubiquitin-protein conjugates. Here, we investigate the effect of surfactants on the efficiency of CuAAC for chemical protein ubiquitylation. We found that anionic surfactants enhance conjugate formation by up to 10-fold resulting in high yields even at low (i.e., micromolar) concentrations of the reactants. Notably, the herein investigated conjugates are functional and thus properly folded.
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SCHNEIDER, Daniel, Tatjana SCHNEIDER, Joos ASCHENBRENNER, Franziska MORTENSEN, Martin SCHEFFNER, Andreas MARX, 2016. Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin. In: Bioorganic & Medicinal Chemistry. 24(5), pp. 995-1001. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2016.01.027BibTex
@article{Schneider2016-03Anion-33623, year={2016}, doi={10.1016/j.bmc.2016.01.027}, title={Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin}, number={5}, volume={24}, issn={0968-0896}, journal={Bioorganic & Medicinal Chemistry}, pages={995--1001}, author={Schneider, Daniel and Schneider, Tatjana and Aschenbrenner, Joos and Mortensen, Franziska and Scheffner, Martin and Marx, Andreas} }
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