Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin

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Bioorganic & Medicinal Chemistry. 2016, 24(5), pp. 995-1001. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2016.01.027
Zusammenfassung

The Cu(I)-catalyzed alkyne-azide cycloaddition (CuAAC) has become increasingly important in the conjugation chemistry of biomolecules. For example, it is an efficient and convenient method to generate defined ubiquitin-protein conjugates. Here, we investigate the effect of surfactants on the efficiency of CuAAC for chemical protein ubiquitylation. We found that anionic surfactants enhance conjugate formation by up to 10-fold resulting in high yields even at low (i.e., micromolar) concentrations of the reactants. Notably, the herein investigated conjugates are functional and thus properly folded.

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ISO 690SCHNEIDER, Daniel, Tatjana SCHNEIDER, Joos ASCHENBRENNER, Franziska MORTENSEN, Martin SCHEFFNER, Andreas MARX, 2016. Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin. In: Bioorganic & Medicinal Chemistry. 2016, 24(5), pp. 995-1001. ISSN 0968-0896. eISSN 1464-3391. Available under: doi: 10.1016/j.bmc.2016.01.027
BibTex
@article{Schneider2016-03Anion-33623,
  year={2016},
  doi={10.1016/j.bmc.2016.01.027},
  title={Anionic surfactants enhance click reaction-mediated protein conjugation with ubiquitin},
  number={5},
  volume={24},
  issn={0968-0896},
  journal={Bioorganic & Medicinal Chemistry},
  pages={995--1001},
  author={Schneider, Daniel and Schneider, Tatjana and Aschenbrenner, Joos and Mortensen, Franziska and Scheffner, Martin and Marx, Andreas}
}
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