## Cellular interaction of the smut fungus Usfacysfis waldsfeiniae

1995
##### Authors
Bauer, Robert
Oberwinkler, Franz
Journal article
##### Published in
Canadian Journal of Botany ; 73 (1995), 6. - pp. 867-883. - ISSN 0008-4026. - eISSN 1480-3305
##### Abstract
The cellular interaction between the smut fungus Ustacystis waldsteiniae and its host Waldsteinia geoides was analyzed by serial-section electron microscopy using chemically fixed and high-pressure frozen -freeze-substituted samples. After penetration, each haustorium extends a short distance into the host cell where it often forms up to three short lobes. The haustorium is wholly ensheathed by a prominent matrix. The matrix is a complex structure, differing significantly from that known of other fungal plant parasites: it is filled with amorphous, electron-opaque material in which membrane-bounded, coralloid vesicles are embedded. During the contact phase of the hypha with the host cell wall, vesicles with electron-opaque contents accumulate in the contact area of the hypha where they appear to fuse with the fungal plasma membrane and extrude their contents. Subsequently, the host cell wall increases in electron opacity and matrix material becomes deposited between host plasma membrane and host cell wall exactly at the ends of the altered areas in the host cell wall. The coralloid vesicles within the matrix, however, are of host origin: exocytosis of Golgi products into tht;: matrix results in the formation of coralloid vesicular buds in the host plasma membrane. Subsequently, the buds seem to detach from the host plasma membrane to flow as coralloid vesicles into the matrix. Matrix development continues during penetration and after penetration at the haustorial tips. After host wall penetration, the fungal cell wall comes in contact with the matrix. The fungal component of the matrix may play a key role in the inducement of these transfer cell-like compartments in host cells responding to infection.
##### Subject (DDC)
570 Biosciences, Biology
##### Keywords
freeze substitution,haustoria,high-pressure freezing,host -parasite interaction,smut fungi,Ustacystis waldsteiniae
##### Cite This
ISO 690BAUER, Robert, Kurt MENDGEN, Franz OBERWINKLER, 1995. Cellular interaction of the smut fungus Usfacysfis waldsfeiniae. In: Canadian Journal of Botany. 73(6), pp. 867-883. ISSN 0008-4026. eISSN 1480-3305. Available under: doi: 10.1139/b95-095
BibTex
@article{Bauer1995Cellu-8066,
year={1995},
doi={10.1139/b95-095},
title={Cellular interaction of the smut fungus Usfacysfis waldsfeiniae},
number={6},
volume={73},
issn={0008-4026},
pages={867--883},
author={Bauer, Robert and Mendgen, Kurt and Oberwinkler, Franz}
}

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<dcterms:abstract xml:lang="eng">The cellular interaction between the smut fungus Ustacystis waldsteiniae and its host Waldsteinia geoides was analyzed by serial-section electron microscopy using chemically fixed and high-pressure frozen -freeze-substituted samples. After penetration, each haustorium extends a short distance into the host cell where it often forms up to three short lobes. The haustorium is wholly ensheathed by a prominent matrix. The matrix is a complex structure, differing significantly from that known of other fungal plant parasites: it is filled with amorphous, electron-opaque material in which membrane-bounded, coralloid vesicles are embedded. During the contact phase of the hypha with the host cell wall, vesicles with electron-opaque contents accumulate in the contact area of the hypha where they appear to fuse with the fungal plasma membrane and extrude their contents. Subsequently, the host cell wall increases in electron opacity and matrix material becomes deposited between host plasma membrane and host cell wall exactly at the ends of the altered areas in the host cell wall. The coralloid vesicles within the matrix, however, are of host origin: exocytosis of Golgi products into tht;: matrix results in the formation of coralloid vesicular buds in the host plasma membrane. Subsequently, the buds seem to detach from the host plasma membrane to flow as coralloid vesicles into the matrix. Matrix development continues during penetration and after penetration at the haustorial tips. After host wall penetration, the fungal cell wall comes in contact with the matrix. The fungal component of the matrix may play a key role in the inducement of these transfer cell-like compartments in host cells responding to infection.</dcterms:abstract>
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