Publikation:

Proton diet for the sodium pump

Lade...
Vorschaubild

Dateien

Apell_Proton.pdf
Apell_Proton.pdfGröße: 1.4 MBDownloads: 401

Datum

2011

Autor:innen

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

DOI (zitierfähiger Link)
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Biochemistry. 2011, 50(3), pp. 409-418. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi101576s

Zusammenfassung

In the absence of Na+ and K+ions the Na,K-ATPase shows a pH-dependent ATP hydrolysis that can be inhibited by ouabain. At pH 7.2 this activity is 5% of the maximal under physiological conditions. It could be inferred that this activity is associated withH þ transport in both directions across the membrane and facilitates an H-only mode of the sodium pump under such unphysiological conditions. By the analysis of experiments with reconstituted proteoliposomes an overall electroneutral transport mode has been proven. The stoichiometry was determined to be 2 H+ /2 H+ /1 ATP and is comparable to what is known from the closely related H,K-ATPase. By time-resolved ATP-concentration jump experiments it was found that at no time was the third, Na þ -specific binding site of the pump occupied by protons.Amodified Post-Albers pump cycle is proposed, with H+ ions as congeners for Na+ and K+, by which all experiments performed can be explained.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690APELL, Hans-Jürgen, Gabriela BENZ, Daniel SAUERBRUNN, 2011. Proton diet for the sodium pump. In: Biochemistry. 2011, 50(3), pp. 409-418. ISSN 0006-2960. eISSN 1520-4995. Available under: doi: 10.1021/bi101576s
BibTex
@article{Apell2011-01-25Proto-14095,
  year={2011},
  doi={10.1021/bi101576s},
  title={Proton diet for the sodium pump},
  number={3},
  volume={50},
  issn={0006-2960},
  journal={Biochemistry},
  pages={409--418},
  author={Apell, Hans-Jürgen and Benz, Gabriela and Sauerbrunn, Daniel}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/14095">
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/14095"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Benz, Gabriela</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>2011-01-25</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14095/2/Apell_Proton.pdf"/>
    <dc:contributor>Sauerbrunn, Daniel</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-07-26T05:33:06Z</dc:date>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Sauerbrunn, Daniel</dc:creator>
    <dc:creator>Benz, Gabriela</dc:creator>
    <dc:creator>Apell, Hans-Jürgen</dc:creator>
    <dc:contributor>Apell, Hans-Jürgen</dc:contributor>
    <dcterms:title>Proton diet for the sodium pump</dcterms:title>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-01-30T23:25:14Z</dcterms:available>
    <dcterms:bibliographicCitation>First publ. in: Biochemistry 50 (2011), 3, S. 409-418</dcterms:bibliographicCitation>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/14095/2/Apell_Proton.pdf"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">In the absence of Na+ and K+ions the Na,K-ATPase shows a pH-dependent ATP hydrolysis that can be inhibited by ouabain. At pH 7.2 this activity is 5% of the maximal under physiological conditions. It could be inferred that this activity is associated withH þ transport in both directions across the membrane and facilitates an H-only mode of the sodium pump under such unphysiological conditions. By the analysis of experiments with reconstituted proteoliposomes an overall electroneutral transport mode has been proven. The stoichiometry was determined to be 2 H+ /2 H+ /1 ATP and is comparable to what is known from the closely related H,K-ATPase. By time-resolved ATP-concentration jump experiments it was found that at no time was the third, Na þ -specific binding site of the pump occupied by protons.Amodified Post-Albers pump cycle is proposed, with H+ ions as congeners for Na+ and K+, by which all experiments performed can be explained.</dcterms:abstract>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen