Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen

Kurzanzeige
dc.contributor.authorScheffner, Martin
dc.contributor.authorWessel, Rainer
dc.contributor.authorStahl, Hans
dc.date.accessioned2018-07-11T09:59:06Z
dc.date.available2018-07-11T09:59:06Z
dc.date.issued1989eng
dc.description.abstractSimian virus 40 (SV40) large tumor antigen (T antigen) is mainly localized in the nucleus where it exhibits two biochemical properties: DNA binding and helicase activity. Both activities are necessary for viral DNA replication and may also enable T antigen to modulate cellular growth. Here we present biochemical and electron microscopic evidence that the helicase activity can start at internal sites of fully double-stranded DNA molecules not containing the SV40 origin or replication. Using T antigen specific monoclonal antibodies, this unwinding reaction can be biochemically divided in an initiation (duplex opening) and a propagation step. The duplex opening reaction (as well as the propagation step) does not depend on a specific DNA sequence or secondary structure. In addition, we have found that T antigen forms an ATP dependent nucleoprotein complex at double-stranded DNA, which may be an essential step for the sequence independent duplex DNA opening reaction.eng
dc.description.versionpublishedeng
dc.identifier.doi10.1093/nar/17.1.93eng
dc.identifier.ppn507498704
dc.identifier.urihttps://kops.uni-konstanz.de/handle/123456789/42843
dc.language.isoengeng
dc.rightsterms-of-use
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dc.subject.ddc570eng
dc.titleSequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigeneng
dc.typeJOURNAL_ARTICLEeng
dspace.entity.typePublication
kops.citation.bibtex
@article{Scheffner1989Seque-42843,
  year={1989},
  doi={10.1093/nar/17.1.93},
  title={Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen},
  number={1},
  volume={17},
  issn={0301-5610},
  journal={Nucleic Acids Research},
  pages={93--106},
  author={Scheffner, Martin and Wessel, Rainer and Stahl, Hans}
}
kops.citation.iso690SCHEFFNER, Martin, Rainer WESSEL, Hans STAHL, 1989. Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen. In: Nucleic Acids Research. 1989, 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93deu
kops.citation.iso690SCHEFFNER, Martin, Rainer WESSEL, Hans STAHL, 1989. Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen. In: Nucleic Acids Research. 1989, 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93eng
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kops.sourcefieldNucleic Acids Research. 1989, <b>17</b>(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93deu
kops.sourcefield.plainNucleic Acids Research. 1989, 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93deu
kops.sourcefield.plainNucleic Acids Research. 1989, 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93eng
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source.periodicalTitleNucleic Acids Researcheng

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