Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen

Scheffner, Martin; Wessel, Rainer; Stahl, Hans

Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen

Dateien

Scheffner_2-f8cp9lug6vhd7.pdf(3.06 MB)

Datum

1989

Autor:innen

Scheffner, Martin

Wessel, Rainer

Stahl, Hans

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Nucleic Acids Research ; 17 (1989), 1. - S. 93-106. - ISSN 0301-5610. - eISSN 1362-4962

Zusammenfassung

Simian virus 40 (SV40) large tumor antigen (T antigen) is mainly localized in the nucleus where it exhibits two biochemical properties: DNA binding and helicase activity. Both activities are necessary for viral DNA replication and may also enable T antigen to modulate cellular growth. Here we present biochemical and electron microscopic evidence that the helicase activity can start at internal sites of fully double-stranded DNA molecules not containing the SV40 origin or replication. Using T antigen specific monoclonal antibodies, this unwinding reaction can be biochemically divided in an initiation (duplex opening) and a propagation step. The duplex opening reaction (as well as the propagation step) does not depend on a specific DNA sequence or secondary structure. In addition, we have found that T antigen forms an ATP dependent nucleoprotein complex at double-stranded DNA, which may be an essential step for the sequence independent duplex DNA opening reaction.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

SCHEFFNER, Martin, Rainer WESSEL, Hans STAHL, 1989. Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen. In: Nucleic Acids Research. 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93

BibTex

@article{Scheffner1989Seque-42843,
  year={1989},
  doi={10.1093/nar/17.1.93},
  title={Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen},
  number={1},
  volume={17},
  issn={0301-5610},
  journal={Nucleic Acids Research},
  pages={93--106},
  author={Scheffner, Martin and Wessel, Rainer and Stahl, Hans}
}

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