Publikation:

Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen

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1989

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Wessel, Rainer
Stahl, Hans

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Nucleic Acids Research. 1989, 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93

Zusammenfassung

Simian virus 40 (SV40) large tumor antigen (T antigen) is mainly localized in the nucleus where it exhibits two biochemical properties: DNA binding and helicase activity. Both activities are necessary for viral DNA replication and may also enable T antigen to modulate cellular growth. Here we present biochemical and electron microscopic evidence that the helicase activity can start at internal sites of fully double-stranded DNA molecules not containing the SV40 origin or replication. Using T antigen specific monoclonal antibodies, this unwinding reaction can be biochemically divided in an initiation (duplex opening) and a propagation step. The duplex opening reaction (as well as the propagation step) does not depend on a specific DNA sequence or secondary structure. In addition, we have found that T antigen forms an ATP dependent nucleoprotein complex at double-stranded DNA, which may be an essential step for the sequence independent duplex DNA opening reaction.

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570 Biowissenschaften, Biologie

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ISO 690SCHEFFNER, Martin, Rainer WESSEL, Hans STAHL, 1989. Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen. In: Nucleic Acids Research. 1989, 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93
BibTex
@article{Scheffner1989Seque-42843,
  year={1989},
  doi={10.1093/nar/17.1.93},
  title={Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen},
  number={1},
  volume={17},
  issn={0301-5610},
  journal={Nucleic Acids Research},
  pages={93--106},
  author={Scheffner, Martin and Wessel, Rainer and Stahl, Hans}
}
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