Publikation:

DafA cycles between the DnaK chaperone system and translational machinery

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2004

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Dumitru, Georgeta L.
Groemping, Yvonne
Klostermeier, Dagmar
Restle, Tobias
Reinstein, Jochen

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Open Access-Veröffentlichung
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Published

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Journal of Molecular Biology. 2004, 339(5), pp. 1179-1189. ISSN 0022-2836. Available under: doi: 10.1016/j.jmb.2004.04.052

Zusammenfassung

DafA is encoded by the dnaK operon of Thermus thermophilus and mediates the formation of a highly stable complex between the chaperone DnaK and its co-chaperone DnaJ under normal growth conditions. DafATth contains 87 amino acid residues and is the only member of the DnaKTth chaperone system for which no corresponding protein has yet been identified in other organisms and whose particular function has remained elusive. Here, we show directly that the DnaKTth DnaJTth DafATth complex cannot represent the active chaperone species since DafATth inhibits renaturation of firefly luciferase by suppressing substrate association. Since DafATth must be released before the substrate proteins can bind we hypothesized that free DafATth might have regulatory functions connected to the heat shock response. Here, we present evidence that supports this hypothesis. We identified the 70 S ribosome as binding target of free DafATth. Our results show that the association of DafATth and 70 S ribosomes does not require the participation of DnaKTth or DnaJTth. On the contrary, the assembly of DnaKTth DnaJTth DafATth and ribosome DafATth complexes seems to be competitive. These findings strongly suggest the involvement of DafATth in regulatory processes occurring at a translational level, which could represent a new mechanism of heat shock response as an adaptation to elevated temperature.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

chaperone, heat shock, ribosome, folding, regulation

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ISO 690DUMITRU, Georgeta L., Yvonne GROEMPING, Dagmar KLOSTERMEIER, Tobias RESTLE, Elke DEUERLING, Jochen REINSTEIN, 2004. DafA cycles between the DnaK chaperone system and translational machinery. In: Journal of Molecular Biology. 2004, 339(5), pp. 1179-1189. ISSN 0022-2836. Available under: doi: 10.1016/j.jmb.2004.04.052
BibTex
@article{Dumitru2004cycle-7940,
  year={2004},
  doi={10.1016/j.jmb.2004.04.052},
  title={DafA cycles between the DnaK chaperone system and translational machinery},
  number={5},
  volume={339},
  issn={0022-2836},
  journal={Journal of Molecular Biology},
  pages={1179--1189},
  author={Dumitru, Georgeta L. and Groemping, Yvonne and Klostermeier, Dagmar and Restle, Tobias and Deuerling, Elke and Reinstein, Jochen}
}
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