Publikation:

N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium

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2002

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Froissard, Marine
Dedieu, Jean-Claude
Gulik-Krzywicki, Tadeusz
Cohen, Jean

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Molekulare Spezifität von Membran-Interaktionen bei Paramecium
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Open Access Green
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Genetics. 2002, 161, pp. 643-650

Zusammenfassung

In exocytosis, secretory granules contact plasmamembrane at sites where microdomains can be observed, which are sometimes marked by intramembranous particle arrays. Such arrays are particularly obvious when membrane fusion is frozen at a subterminal stage, e.g., in neuromuscular junctions and ciliate exocytotic sites. In Paramecium, a genetic approach has shown thatthe rosettes of intramembranous particles are essential for stimulated exocytosis of secretory granules, the trichocysts. The identification of two genes encoding the N-ethylmaleimide-sensitive factor (NSF), a chaperone ATPase involved in organelle docking, prompted us to analyze its potential role in trichocyst exocytosis using a gene-silencing strategy. Here we show that NSF deprivation strongly interferes with rosette assembly but does not disturb the functioning of exocytotic sites already formed. We conclude that rosette organization involves ubiquitous partners of the fusion machinery and discuss where NSF could intervene in this mechanism.

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570 Biowissenschaften, Biologie

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ISO 690FROISSARD, Marine, Roland KISSMEHL, Jean-Claude DEDIEU, Tadeusz GULIK-KRZYWICKI, Helmut PLATTNER, Jean COHEN, 2002. N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium. In: Genetics. 2002, 161, pp. 643-650
BibTex
@article{Froissard2002NEthy-7848,
  year={2002},
  title={N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium},
  volume={161},
  journal={Genetics},
  pages={643--650},
  author={Froissard, Marine and Kissmehl, Roland and Dedieu, Jean-Claude and Gulik-Krzywicki, Tadeusz and Plattner, Helmut and Cohen, Jean}
}
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