N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium
N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium
Loading...
Date
2002
Authors
Froissard, Marine
Dedieu, Jean-Claude
Gulik-Krzywicki, Tadeusz
Cohen, Jean
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
International patent number
Link to the license
EU project number
Project
Molekulare Spezifität von Membran-Interaktionen bei Paramecium
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
Genetics ; 161 (2002). - pp. 643-650
Abstract
In exocytosis, secretory granules contact plasmamembrane at sites where microdomains can be observed, which are sometimes marked by intramembranous particle arrays. Such arrays are particularly obvious when membrane fusion is frozen at a subterminal stage, e.g., in neuromuscular junctions and ciliate exocytotic sites. In Paramecium, a genetic approach has shown thatthe rosettes of intramembranous particles are essential for stimulated exocytosis of secretory granules, the trichocysts. The identification of two genes encoding the N-ethylmaleimide-sensitive factor (NSF), a chaperone ATPase involved in organelle docking, prompted us to analyze its potential role in trichocyst exocytosis using a gene-silencing strategy. Here we show that NSF deprivation strongly interferes with rosette assembly but does not disturb the functioning of exocytotic sites already formed. We conclude that rosette organization involves ubiquitous partners of the fusion machinery and discuss where NSF could intervene in this mechanism.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
FROISSARD, Marine, Roland KISSMEHL, Jean-Claude DEDIEU, Tadeusz GULIK-KRZYWICKI, Helmut PLATTNER, Jean COHEN, 2002. N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium. In: Genetics. 161, pp. 643-650BibTex
@article{Froissard2002NEthy-7848, year={2002}, title={N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium}, volume={161}, journal={Genetics}, pages={643--650}, author={Froissard, Marine and Kissmehl, Roland and Dedieu, Jean-Claude and Gulik-Krzywicki, Tadeusz and Plattner, Helmut and Cohen, Jean} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7848"> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7848/1/Factor_Is_Required_to_Organize_Functional.pdf"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7848/1/Factor_Is_Required_to_Organize_Functional.pdf"/> <dc:contributor>Dedieu, Jean-Claude</dc:contributor> <dcterms:title>N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium</dcterms:title> <dc:contributor>Plattner, Helmut</dc:contributor> <dcterms:issued>2002</dcterms:issued> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7848"/> <dc:language>eng</dc:language> <dc:contributor>Cohen, Jean</dc:contributor> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dcterms:abstract xml:lang="eng">In exocytosis, secretory granules contact plasmamembrane at sites where microdomains can be observed, which are sometimes marked by intramembranous particle arrays. Such arrays are particularly obvious when membrane fusion is frozen at a subterminal stage, e.g., in neuromuscular junctions and ciliate exocytotic sites. In Paramecium, a genetic approach has shown thatthe rosettes of intramembranous particles are essential for stimulated exocytosis of secretory granules, the trichocysts. The identification of two genes encoding the N-ethylmaleimide-sensitive factor (NSF), a chaperone ATPase involved in organelle docking, prompted us to analyze its potential role in trichocyst exocytosis using a gene-silencing strategy. Here we show that NSF deprivation strongly interferes with rosette assembly but does not disturb the functioning of exocytotic sites already formed. We conclude that rosette organization involves ubiquitous partners of the fusion machinery and discuss where NSF could intervene in this mechanism.</dcterms:abstract> <dc:contributor>Kissmehl, Roland</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Froissard, Marine</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:59Z</dcterms:available> <dc:creator>Froissard, Marine</dc:creator> <dc:creator>Plattner, Helmut</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <dc:creator>Cohen, Jean</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:59Z</dc:date> <dcterms:bibliographicCitation>First publ. in: Genetics ; 161 (2002). - S. 643-650</dcterms:bibliographicCitation> <dc:format>application/pdf</dc:format> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Gulik-Krzywicki, Tadeusz</dc:contributor> <dc:creator>Kissmehl, Roland</dc:creator> <dc:creator>Dedieu, Jean-Claude</dc:creator> <dc:creator>Gulik-Krzywicki, Tadeusz</dc:creator> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No