Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri

Pomowski, Anja; Zumft, Walter G.; Kroneck, Peter M. H.; Einsle, Oliver

Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri

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Date

2010

Authors

Pomowski, Anja

Zumft, Walter G.

Kroneck, Peter M. H.

Einsle, Oliver

DOI (citable link)

10.1107/S1744309110038832

Collections

Biologie

Publication type

Journal article

Publication status

Published

Published in

Acta Crystallographica / Section F Structural Biology and Crystallization Communications ; 66 (2010), 11. - pp. 1541-1543. - ISSN 1744-3091. - eISSN 2053-230X

Abstract

Nitrous oxide reductase (N₂OR) from Pseudomonas stutzeri catalyzes the final step in denitrification: the two-electron reduction of nitrous oxide to molecular dinitrogen. Crystals of the enzyme were grown under strict exclusion of dioxygen by sitting-drop vapour diffusion using 2R,3R-butanediol as a cryoprotectant. N₂OR crystallized in either space group P1 or P6₅. Interestingly, the key determinant for the resulting space group was the crystallization temperature. Crystals belonging to space group P1 contained four 130 kDa dimers in the asymmetric unit, while crystals belonging to space group P6₅ contained a single dimer in the asymmetric unit. Diffraction data were collected to resolutions better than 2 Å.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

POMOWSKI, Anja, Walter G. ZUMFT, Peter M. H. KRONECK, Oliver EINSLE, 2010. Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri. In: Acta Crystallographica / Section F Structural Biology and Crystallization Communications. 66(11), pp. 1541-1543. ISSN 1744-3091. eISSN 2053-230X. Available under: doi: 10.1107/S1744309110038832

BibTex

@article{Pomowski2010-11-01Cryst-35807,
  year={2010},
  doi={10.1107/S1744309110038832},
  title={Crystallization of purple nitrous oxide reductase from Pseudomonas stutzeri},
  number={11},
  volume={66},
  issn={1744-3091},
  journal={Acta Crystallographica / Section F Structural Biology and Crystallization Communications},
  pages={1541--1543},
  author={Pomowski, Anja and Zumft, Walter G. and Kroneck, Peter M. H. and Einsle, Oliver}
}

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    <dcterms:abstract xml:lang="eng">Nitrous oxide reductase (N&lt;sub&gt;2&lt;/sub&gt;OR) from Pseudomonas stutzeri catalyzes the final step in denitrification: the two-electron reduction of nitrous oxide to molecular dinitrogen. Crystals of the enzyme were grown under strict exclusion of dioxygen by sitting-drop vapour diffusion using 2R,3R-butanediol as a cryoprotectant. N&lt;sub&gt;2&lt;/sub&gt;OR crystallized in either space group P1 or P6&lt;sub&gt;5&lt;/sub&gt;. Interestingly, the key determinant for the resulting space group was the crystallization temperature. Crystals belonging to space group P1 contained four 130 kDa dimers in the asymmetric unit, while crystals belonging to space group P6&lt;sub&gt;5&lt;/sub&gt; contained a single dimer in the asymmetric unit. Diffraction data were collected to resolutions better than 2 Å.</dcterms:abstract>
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Bibliography of Konstanz

Yes