Publikation: Spheroids formation in large drops suspended in superhydrophobic paper cones
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
The utilization of 3D cell culture for spheroid formation holds significant implications in cancer research, contributing to a fundamental understanding of the disease and aiding drug development. Conventional methods such as the hanging drop technique and other alternatives encounter limitations due to smaller drop volumes, leading to nutrient starvation and restricted culture duration. In this study, we present a straightforward approach to creating superhydrophobic paper cones capable of accommodating large volumes of culture media drops. These paper cones have sterility, autoclavability, and bacterial repellent properties. Leveraging these attributes, we successfully generate large spheroids of ovarian cancer cells and, as a proof of concept, conduct drug screening to assess the impact of carboplatin. Thus, our method enables the preparation of flexible superhydrophobic surfaces for laboratory applications in an expeditious manner, exemplified here through spheroid formation and drug screening demonstrations.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
MOHAPATRA, Omkar, Maheshwar Reddy GOPU, Rahail ASHRAF, Jijo EASO GEORGE, Saniya PATIL, Raju MUKHERJEE, Sanjay VINOD KUMAR, Dileep MAMPALLIL, 2024. Spheroids formation in large drops suspended in superhydrophobic paper cones. In: Biomicrofluidics. AIP Publishing. 2024, 18(2), 024107. eISSN 1932-1058. Verfügbar unter: doi: 10.1063/5.0197807BibTex
@article{Mohapatra2024-03-01Spher-71799, year={2024}, doi={10.1063/5.0197807}, title={Spheroids formation in large drops suspended in superhydrophobic paper cones}, number={2}, volume={18}, journal={Biomicrofluidics}, author={Mohapatra, Omkar and Gopu, Maheshwar Reddy and Ashraf, Rahail and Easo George, Jijo and Patil, Saniya and Mukherjee, Raju and Vinod Kumar, Sanjay and Mampallil, Dileep}, note={Article Number: 024107} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/71799"> <dc:contributor>Mampallil, Dileep</dc:contributor> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:creator>Mukherjee, Raju</dc:creator> <dc:creator>Easo George, Jijo</dc:creator> <dc:contributor>Vinod Kumar, Sanjay</dc:contributor> <dc:creator>Mampallil, Dileep</dc:creator> <dc:language>eng</dc:language> <dc:contributor>Mohapatra, Omkar</dc:contributor> <dc:contributor>Easo George, Jijo</dc:contributor> <dc:contributor>Mukherjee, Raju</dc:contributor> <dc:contributor>Ashraf, Rahail</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2025-01-08T12:02:28Z</dcterms:available> <dcterms:abstract>The utilization of 3D cell culture for spheroid formation holds significant implications in cancer research, contributing to a fundamental understanding of the disease and aiding drug development. Conventional methods such as the hanging drop technique and other alternatives encounter limitations due to smaller drop volumes, leading to nutrient starvation and restricted culture duration. In this study, we present a straightforward approach to creating superhydrophobic paper cones capable of accommodating large volumes of culture media drops. These paper cones have sterility, autoclavability, and bacterial repellent properties. Leveraging these attributes, we successfully generate large spheroids of ovarian cancer cells and, as a proof of concept, conduct drug screening to assess the impact of carboplatin. Thus, our method enables the preparation of flexible superhydrophobic surfaces for laboratory applications in an expeditious manner, exemplified here through spheroid formation and drug screening demonstrations.</dcterms:abstract> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/71799"/> <dc:creator>Vinod Kumar, Sanjay</dc:creator> <dcterms:title>Spheroids formation in large drops suspended in superhydrophobic paper cones</dcterms:title> <dc:creator>Mohapatra, Omkar</dc:creator> <dc:creator>Gopu, Maheshwar Reddy</dc:creator> <dc:contributor>Gopu, Maheshwar Reddy</dc:contributor> <dc:contributor>Patil, Saniya</dc:contributor> <dc:creator>Ashraf, Rahail</dc:creator> <dcterms:issued>2024-03-01</dcterms:issued> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2025-01-08T12:02:28Z</dc:date> <dc:creator>Patil, Saniya</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/41"/> </rdf:Description> </rdf:RDF>