TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses

Schlosser, Eva Christine; Müller, Marc; Fischer, Stefan; Basta, Sameh; Busch, Dirk H.; Gander, Bruno; Gröttrup, Marcus

TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses

Files

Schlosser_opus-85078.pdf(652.1 KB)

Date

2008

Authors

Schlosser, Eva Christine

Müller, Marc

Fischer, Stefan

Basta, Sameh

Busch, Dirk H.

Gander, Bruno

Gröttrup, Marcus

Publication type

Journal article

Published in

Vaccine ; 26 (2008), 13. - pp. 1626-1637. - ISSN 0264-410X

Abstract

Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(D,L-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9% SIINFEKL/H-2K(b) tetramer positive CTLs, production of IFN-gamma, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.

Subject (DDC)

570 Biosciences, Biology

Cite This

ISO 690

SCHLOSSER, Eva Christine, Marc MÜLLER, Stefan FISCHER, Sameh BASTA, Dirk H. BUSCH, Bruno GANDER, Marcus GRÖTTRUP, 2008. TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses. In: Vaccine. 26(13), pp. 1626-1637. ISSN 0264-410X. Available under: doi: 10.1016/j.vaccine.2008.01.030

BibTex

@article{Schlosser2008ligan-1194,
  year={2008},
  doi={10.1016/j.vaccine.2008.01.030},
  title={TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses},
  number={13},
  volume={26},
  issn={0264-410X},
  journal={Vaccine},
  pages={1626--1637},
  author={Schlosser, Eva Christine and Müller, Marc and Fischer, Stefan and Basta, Sameh and Busch, Dirk H. and Gander, Bruno and Gröttrup, Marcus}
}

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Bibliography of Konstanz

Yes