TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses

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2008
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Schlosser, Eva Christine
Fischer, Stefan
Basta, Sameh
Busch, Dirk H.
Gander, Bruno
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Vaccine. 2008, 26(13), pp. 1626-1637. ISSN 0264-410X. Available under: doi: 10.1016/j.vaccine.2008.01.030
Zusammenfassung

Dendritic cells phagocytose pathogens leading to maturation and cross-presentation on MHC class I. We found that the efficiency of cross-priming in mice after vaccination with biodegradable poly(D,L-lactide-co-glycolide) microspheres (MSs) was enhanced when ovalbumin was coencapsulated together with either a CpG oligonucleotide or polyI:C as compared to co-inoculation of ovalbumin-bearing MS with soluble or separately encapsulated adjuvants. A single immunization with MS containing coencaspsulated CpG and ovalbumin yielded 9% SIINFEKL/H-2K(b) tetramer positive CTLs, production of IFN-gamma, efficient cytolysis, and protection from vaccinia virus infection. Taken together, coencapsulation of adjuvant and antigen is an important paradigm for the generation of potent CTL responses.

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ISO 690SCHLOSSER, Eva Christine, Marc MÜLLER, Stefan FISCHER, Sameh BASTA, Dirk H. BUSCH, Bruno GANDER, Marcus GRÖTTRUP, 2008. TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses. In: Vaccine. 2008, 26(13), pp. 1626-1637. ISSN 0264-410X. Available under: doi: 10.1016/j.vaccine.2008.01.030
BibTex
@article{Schlosser2008ligan-1194,
  year={2008},
  doi={10.1016/j.vaccine.2008.01.030},
  title={TLR ligands and antigen need to be coencapsulated into the same biodegradable microsphere for the generation of potent cytotoxic T lymphocyte responses},
  number={13},
  volume={26},
  issn={0264-410X},
  journal={Vaccine},
  pages={1626--1637},
  author={Schlosser, Eva Christine and Müller, Marc and Fischer, Stefan and Basta, Sameh and Busch, Dirk H. and Gander, Bruno and Gröttrup, Marcus}
}
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