Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27

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2008
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Acta Crystallographica Section F Structural Biology and Crystallization Communications. 2008, 64(6), pp. 533-536. eISSN 1744-3091. Available under: doi: 10.1107/S1744309108013602
Zusammenfassung

The cell envelope of the thermophilic bacterium Thermus thermophilus is multilayered and includes an outer membrane with integral outer membrane proteins that are not well characterized. The hypothetical protein TTC0834 from T. thermophilus HB27 was identified as a 22 kDa outer membrane protein containing eight predicted -strands. TTC0834 was expressed with an N-terminal His tag in T. thermophilus HB8 and detected in the S-layer/outer membrane envelope fraction. His-TTC0834 was purified and crystallized under various conditions. Native data sets were collected to 3.2 Å resolution and the best diffracting crystals belonged to space group P3121 or P3221, with unit-cell parameters a = b = 166.67, c = 97.53 Å.

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570 Biowissenschaften, Biologie
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Thermus thermophilus, outer membrane protein, TTC0834
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ISO 690BROSIG, Alexander, Jutta NESPER, Wolfram WELTE, Kay DIEDERICHS, 2008. Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27. In: Acta Crystallographica Section F Structural Biology and Crystallization Communications. 2008, 64(6), pp. 533-536. eISSN 1744-3091. Available under: doi: 10.1107/S1744309108013602
BibTex
@article{Brosig2008-06-01Expre-1124,
  year={2008},
  doi={10.1107/S1744309108013602},
  title={Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27},
  number={6},
  volume={64},
  journal={Acta Crystallographica Section F Structural Biology and Crystallization Communications},
  pages={533--536},
  author={Brosig, Alexander and Nesper, Jutta and Welte, Wolfram and Diederichs, Kay}
}
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