Publikation:

Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27

Lade...
Vorschaubild

Dateien

Brosig_117786.pdf
Brosig_117786.pdfGröße: 320.63 KBDownloads: 133

Datum

2008

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Acta Crystallographica Section F Structural Biology and Crystallization Communications. 2008, 64(6), pp. 533-536. eISSN 1744-3091. Available under: doi: 10.1107/S1744309108013602

Zusammenfassung

The cell envelope of the thermophilic bacterium Thermus thermophilus is multilayered and includes an outer membrane with integral outer membrane proteins that are not well characterized. The hypothetical protein TTC0834 from T. thermophilus HB27 was identified as a 22 kDa outer membrane protein containing eight predicted -strands. TTC0834 was expressed with an N-terminal His tag in T. thermophilus HB8 and detected in the S-layer/outer membrane envelope fraction. His-TTC0834 was purified and crystallized under various conditions. Native data sets were collected to 3.2 Å resolution and the best diffracting crystals belonged to space group P3121 or P3221, with unit-cell parameters a = b = 166.67, c = 97.53 Å.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Thermus thermophilus, outer membrane protein, TTC0834

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690BROSIG, Alexander, Jutta NESPER, Wolfram WELTE, Kay DIEDERICHS, 2008. Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27. In: Acta Crystallographica Section F Structural Biology and Crystallization Communications. 2008, 64(6), pp. 533-536. eISSN 1744-3091. Available under: doi: 10.1107/S1744309108013602
BibTex
@article{Brosig2008-06-01Expre-1124,
  year={2008},
  doi={10.1107/S1744309108013602},
  title={Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27},
  number={6},
  volume={64},
  journal={Acta Crystallographica Section F Structural Biology and Crystallization Communications},
  pages={533--536},
  author={Brosig, Alexander and Nesper, Jutta and Welte, Wolfram and Diederichs, Kay}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/1124">
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">The cell envelope of the thermophilic bacterium Thermus thermophilus is multilayered and includes an outer membrane with integral outer membrane proteins that are not well characterized. The hypothetical protein TTC0834 from T. thermophilus HB27 was identified as a 22 kDa outer membrane protein containing eight predicted -strands. TTC0834 was expressed with an N-terminal His tag in T. thermophilus HB8 and detected in the S-layer/outer membrane envelope fraction. His-TTC0834 was purified and crystallized under various conditions. Native data sets were collected to 3.2 Å resolution and the best diffracting crystals belonged to space group P3121 or P3221, with unit-cell parameters a = b = 166.67, c = 97.53 Å.</dcterms:abstract>
    <dc:contributor>Welte, Wolfram</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:19Z</dc:date>
    <dcterms:issued>2008-06-01</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:language>eng</dc:language>
    <dc:contributor>Brosig, Alexander</dc:contributor>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dc:creator>Brosig, Alexander</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-23T09:06:19Z</dcterms:available>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/1124"/>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Nesper, Jutta</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Welte, Wolfram</dc:creator>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/1124/1/Brosig_117786.pdf"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/1124/1/Brosig_117786.pdf"/>
    <dcterms:bibliographicCitation>Publ. in: Acta Crystallographica / Section F, Structural biology and crystallization communications, 64 (2008), 6, pp. 533-536</dcterms:bibliographicCitation>
    <dc:contributor>Nesper, Jutta</dc:contributor>
    <dcterms:title>Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen