IAPs regulate the plasticity of cell migration by directly targeting Rac1 for degradation

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2012
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Oberoi, Tripat Kaur
Dogan, Taner
Hocking, Jennifer C.
Scholz, Rolf-Peter
Mooz, Juliane
Anderson, Carrie L.
Meyer zu Heringdorf, Dagmar
Schmidt, Gudula
Rajalingam, Krishnaraj
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Zusammenfassung

Inhibitors of apoptosis proteins (IAPs) are a highly conserved class of multifunctional proteins. Rac1 is a well-studied Rho GTPase that controls numerous basic cellular processes. While the regulation of nucleotide binding to Rac1 is well understood, the molecular mechanisms controlling Rac1 degradation are not known. Here, we demonstrate X-linked IAP (XIAP) and cellular IAP1 (c-IAP1) directly bind to Rac1 in a nucleotide-independent manner to promote its polyubiquitination at Lys147 and proteasomal degradation. These IAPs are also required for degradation of Rac1 upon CNF1 toxin treatment or RhoGDI depletion. Consistently, downregulation of XIAP or c-IAP1 by various strategies led to an increase in Rac1 protein levels in primary and tumour cells, leading to an elongated morphology and enhanced cell migration. Further, XIAP counteracts Rac1-dependent cellular polarization in the developing zebrafish hindbrain and promotes the delamination of neurons from the normal tissue architecture. These observations unveil an evolutionarily conserved role of IAPs in controlling Rac1 stability thereby regulating the plasticity of cell migration and morphogenesis.

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570 Biowissenschaften, Biologie
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apoptosis, IAP, Rac1
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ISO 690OBEROI, Tripat Kaur, Taner DOGAN, Jennifer C. HOCKING, Rolf-Peter SCHOLZ, Juliane MOOZ, Carrie L. ANDERSON, Christiaan KARREMAN, Dagmar MEYER ZU HERINGDORF, Gudula SCHMIDT, Krishnaraj RAJALINGAM, 2012. IAPs regulate the plasticity of cell migration by directly targeting Rac1 for degradation. In: The EMBO journal. 2012, 31(1), pp. 14-28. ISSN 0261-4189. eISSN 1460-2075. Available under: doi: 10.1038/emboj.2011.423
BibTex
@article{Oberoi2012-01-04regul-38811,
  year={2012},
  doi={10.1038/emboj.2011.423},
  title={IAPs regulate the plasticity of cell migration by directly targeting Rac1 for degradation},
  number={1},
  volume={31},
  issn={0261-4189},
  journal={The EMBO journal},
  pages={14--28},
  author={Oberoi, Tripat Kaur and Dogan, Taner and Hocking, Jennifer C. and Scholz, Rolf-Peter and Mooz, Juliane and Anderson, Carrie L. and Karreman, Christiaan and Meyer zu Heringdorf, Dagmar and Schmidt, Gudula and Rajalingam, Krishnaraj}
}
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