## In situ detection of heavy metal substituted chlorophylls in water plants

1998
##### Authors
Küpper, Frithjof Christian
Spiller, Martin
Journal article
##### Published in
Photosynthesis Research ; 58 (1998), 2. - pp. 123-133. - ISSN 0166-8595
##### Abstract
The in vivo substitution of magnesium, the central atom of chlorophyll, by heavy metals (mercury, copper, cadmium, nickel, zinc, lead) leads to a breakdown in photosynthesis and is an important damage mechanism in heavy metal-stressed plants. In this study, a number of methods are presented for the efficient in situ detection of this substitution (i.e. in whole plants or in chloroplasts). While macroscopic observations point to the formation of heavy metal chlorophylls at higher concentrations, fluorescence microscopy enables the detection of this reaction at very low substitution rates. Therefore, the course of the reaction can be followed by continuously measuring the fluorescence of whole plants. Furthermore absorbance spectroscopy of whole cells or isolated chloroplasts also enables the in situ detection of heavy metal chlorophylls. These methods provide practicable approaches in detecting the formation of these compounds in situ, avoiding artefacts that might occur using extraction methods based on polar solvents. In addition to the new methods for in situ detection, an extreme heterogeneity in the reaction of cells in the same tissue upon heavy metal stress was observed: while some cells are already disintegrating, others still show normal fluorescence and photosynthetic activity. Measurements of fluorescence kinetics gave a further hint that in high light intensity a substitution of Mg by heavy metals might take place specifically in PS II reaction centres.
##### Subject (DDC)
570 Biosciences, Biology
##### Keywords
antenna pigments,copper,fluorescence microscopy,zinc,fluorimetry,spectroscopy
##### Cite This
ISO 690KÜPPER, Hendrik, Frithjof Christian KÜPPER, Martin SPILLER, 1998. In situ detection of heavy metal substituted chlorophylls in water plants. In: Photosynthesis Research. 58(2), pp. 123-133. ISSN 0166-8595. Available under: doi: 10.1023/A:1006132608181
BibTex
@article{Kupper1998detec-7843,
year={1998},
doi={10.1023/A:1006132608181},
title={In situ detection of heavy metal substituted chlorophylls in water plants},
number={2},
volume={58},
issn={0166-8595},
journal={Photosynthesis Research},
pages={123--133},
author={Küpper, Hendrik and Küpper, Frithjof Christian and Spiller, Martin}
}

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<dcterms:abstract xml:lang="deu">The in vivo substitution of magnesium, the central atom of chlorophyll, by heavy metals (mercury, copper, cadmium, nickel, zinc, lead) leads to a breakdown in photosynthesis and is an important damage mechanism in heavy metal-stressed plants. In this study, a number of methods are presented for the efficient in situ detection of this substitution (i.e. in whole plants or in chloroplasts). While macroscopic observations point to the formation of heavy metal chlorophylls at higher concentrations, fluorescence microscopy enables the detection of this reaction at very low substitution rates. Therefore, the course of the reaction can be followed by continuously measuring the fluorescence of whole plants. Furthermore absorbance spectroscopy of whole cells or isolated chloroplasts also enables the in situ detection of heavy metal chlorophylls. These methods provide practicable approaches in detecting the formation of these compounds in situ, avoiding artefacts that might occur using extraction methods based on polar solvents. In addition to the new methods for in situ detection, an extreme heterogeneity in the reaction of cells in the same tissue upon heavy metal stress was observed: while some cells are already disintegrating, others still show normal fluorescence and photosynthetic activity. Measurements of fluorescence kinetics gave a further hint that in high light intensity a substitution of Mg by heavy metals might take place specifically in PS II reaction centres.</dcterms:abstract>
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