Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization
Dateien
Datum
Autor:innen
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
Internationale Patentnummer
EU-Projektnummer
DFG-Projektnummer
Projekt
Open Access-Veröffentlichung
Sammlungen
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
NIKOLAY, Rainer, Tarek HILAL, Sabine SCHMIDT, Bo QIN, David SCHWEFEL, Carlos H. VIEIRA-VIEIRA, Thorsten MIELKE, Jörg BÜRGER, Elke DEUERLING, Christian M.T. SPAHN, 2021. Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization. In: Molecular Cell. Elsevier. 2021, 81(6), pp. 1200-1215.e9. ISSN 1097-2765. eISSN 1097-4164. Available under: doi: 10.1016/j.molcel.2021.02.006BibTex
@article{Nikolay2021Snaps-53811, year={2021}, doi={10.1016/j.molcel.2021.02.006}, title={Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization}, number={6}, volume={81}, issn={1097-2765}, journal={Molecular Cell}, pages={1200--1215.e9}, author={Nikolay, Rainer and Hilal, Tarek and Schmidt, Sabine and Qin, Bo and Schwefel, David and Vieira-Vieira, Carlos H. and Mielke, Thorsten and Bürger, Jörg and Deuerling, Elke and Spahn, Christian M.T.} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/53811"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-05-28T12:40:55Z</dcterms:available> <dc:contributor>Spahn, Christian M.T.</dc:contributor> <dc:contributor>Schwefel, David</dc:contributor> <dc:contributor>Mielke, Thorsten</dc:contributor> <dc:creator>Nikolay, Rainer</dc:creator> <dcterms:abstract xml:lang="eng">Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.</dcterms:abstract> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Vieira-Vieira, Carlos H.</dc:creator> <dc:contributor>Qin, Bo</dc:contributor> <dcterms:issued>2021</dcterms:issued> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:contributor>Schmidt, Sabine</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-05-28T12:40:55Z</dc:date> <dc:language>eng</dc:language> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:creator>Schwefel, David</dc:creator> <dc:contributor>Hilal, Tarek</dc:contributor> <dc:contributor>Bürger, Jörg</dc:contributor> <dcterms:title>Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization</dcterms:title> <dc:creator>Qin, Bo</dc:creator> <dc:creator>Bürger, Jörg</dc:creator> <dc:creator>Schmidt, Sabine</dc:creator> <dc:creator>Spahn, Christian M.T.</dc:creator> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Vieira-Vieira, Carlos H.</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:contributor>Nikolay, Rainer</dc:contributor> <dc:creator>Deuerling, Elke</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Deuerling, Elke</dc:contributor> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/53811"/> <dc:creator>Mielke, Thorsten</dc:creator> <dc:creator>Hilal, Tarek</dc:creator> </rdf:Description> </rdf:RDF>