Publikation:

Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization

Vorschaubild

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2021

Autor:innen

Hilal, Tarek
Qin, Bo
Schwefel, David
Vieira-Vieira, Carlos H.
Mielke, Thorsten
Bürger, Jörg
Spahn, Christian M.T.
et al.

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
DOI (zitierfähiger Link)
https://doi.org/10.1016/j.molcel.2021.02.006
ArXiv-ID

Internationale Patentnummer

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Molecular Cell. Elsevier. 2021, 81(6), pp. 1200-1215.e9. ISSN 1097-2765. eISSN 1097-4164. Available under: doi: 10.1016/j.molcel.2021.02.006

Zusammenfassung

Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

ribosome, ribosome assembly, ribosome biogenesis, 50S subunit, ObgE, RluD, RsfS, YjgA, cryo-electron microscopy, structure

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690NIKOLAY, Rainer, Tarek HILAL, Sabine SCHMIDT, Bo QIN, David SCHWEFEL, Carlos H. VIEIRA-VIEIRA, Thorsten MIELKE, Jörg BÜRGER, Elke DEUERLING, Christian M.T. SPAHN, 2021. Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization. In: Molecular Cell. Elsevier. 2021, 81(6), pp. 1200-1215.e9. ISSN 1097-2765. eISSN 1097-4164. Available under: doi: 10.1016/j.molcel.2021.02.006
BibTex
@article{Nikolay2021Snaps-53811,
  year={2021},
  doi={10.1016/j.molcel.2021.02.006},
  title={Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization},
  number={6},
  volume={81},
  issn={1097-2765},
  journal={Molecular Cell},
  pages={1200--1215.e9},
  author={Nikolay, Rainer and Hilal, Tarek and Schmidt, Sabine and Qin, Bo and Schwefel, David and Vieira-Vieira, Carlos H. and Mielke, Thorsten and Bürger, Jörg and Deuerling, Elke and Spahn, Christian M.T.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/53811">
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-05-28T12:40:55Z</dcterms:available>
    <dc:contributor>Spahn, Christian M.T.</dc:contributor>
    <dc:contributor>Schwefel, David</dc:contributor>
    <dc:contributor>Mielke, Thorsten</dc:contributor>
    <dc:creator>Nikolay, Rainer</dc:creator>
    <dcterms:abstract xml:lang="eng">Ribosome biogenesis is a fundamental multi-step cellular process that culminates in the formation of ribosomal subunits, whose production and modification are regulated by numerous biogenesis factors. In this study, we analyze physiologic prokaryotic ribosome biogenesis by isolating bona fide pre-50S subunits from an Escherichia coli strain with the biogenesis factor ObgE, affinity tagged at its native gene locus. Our integrative structural approach reveals a network of interacting biogenesis factors consisting of YjgA, RluD, RsfS, and ObgE on the immature pre-50S subunit. In addition, our study provides mechanistic insight into how the GTPase ObgE, in concert with other biogenesis factors, facilitates the maturation of the 50S functional core and reveals both conserved and divergent evolutionary features of ribosome biogenesis between prokaryotes and eukaryotes.</dcterms:abstract>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Vieira-Vieira, Carlos H.</dc:creator>
    <dc:contributor>Qin, Bo</dc:contributor>
    <dcterms:issued>2021</dcterms:issued>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Schmidt, Sabine</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2021-05-28T12:40:55Z</dc:date>
    <dc:language>eng</dc:language>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Schwefel, David</dc:creator>
    <dc:contributor>Hilal, Tarek</dc:contributor>
    <dc:contributor>Bürger, Jörg</dc:contributor>
    <dcterms:title>Snapshots of native pre-50S ribosomes reveal a biogenesis factor network and evolutionary specialization</dcterms:title>
    <dc:creator>Qin, Bo</dc:creator>
    <dc:creator>Bürger, Jörg</dc:creator>
    <dc:creator>Schmidt, Sabine</dc:creator>
    <dc:creator>Spahn, Christian M.T.</dc:creator>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Vieira-Vieira, Carlos H.</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:contributor>Nikolay, Rainer</dc:contributor>
    <dc:creator>Deuerling, Elke</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Deuerling, Elke</dc:contributor>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/53811"/>
    <dc:creator>Mielke, Thorsten</dc:creator>
    <dc:creator>Hilal, Tarek</dc:creator>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Ja
Diese Publikation teilen