Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells

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2006
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Langhorst, Matthias F.
Luxenhofer, Georg
Boneberg, Eva-Maria
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The FASEB Journal. 2006, 20(6), pp. 711-713. ISSN 0892-6638. eISSN 1530-6860. Available under: doi: 10.1096/fj.05-4760fje
Zusammenfassung

T cell activation after contact with an antigen-presenting cell depends on the regulated assembly of the T cell receptor signaling complex, which involves the polarized assembly of a stable, raftlike macrodomain surrounding engaged T cell receptors. Here we show that the preformed reggie/flotillin caps present in resting T cells act as priming platforms for macrodomain assembly. Preformed reggie-1/flotillin-2 caps are exceptionally stable, as shown by fluorescence recovery after photobleaching (FRAP). Upon T cell stimulation, signaling molecules are recruited to the stable reggie/flotillin caps. Importantly, a trans-negative reggie-1/flotillin-2 deletion mutant, which interferes with assembly of the preformed reggie/flotillin cap, impairs raft polarization and macrodomain formation after T cell activation. Accordingly, expression of the trans-negative reggie-1 mutant leads to the incorrect positioning of the guanine nucleotide exchange factor Vav, resulting in defects in cytoskeletal reorganization. Thus, the preformed reggie/flotillin caps are stable priming platforms for the assembly of multiprotein complexes controlling actin reorganization during T cell activation.

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570 Biowissenschaften, Biologie
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lipid rafts, signal transduction, T cell activation, actin cytoskeleton, raft clustering
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ISO 690LANGHORST, Matthias F., Alexander REUTER, Georg LUXENHOFER, Eva-Maria BONEBERG, Daniel F. LEGLER, Helmut PLATTNER, Claudia STÜRMER, 2006. Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells. In: The FASEB Journal. 2006, 20(6), pp. 711-713. ISSN 0892-6638. eISSN 1530-6860. Available under: doi: 10.1096/fj.05-4760fje
BibTex
@article{Langhorst2006-04Prefo-6738,
  year={2006},
  doi={10.1096/fj.05-4760fje},
  title={Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells},
  number={6},
  volume={20},
  issn={0892-6638},
  journal={The FASEB Journal},
  pages={711--713},
  author={Langhorst, Matthias F. and Reuter, Alexander and Luxenhofer, Georg and Boneberg, Eva-Maria and Legler, Daniel F. and Plattner, Helmut and Stürmer, Claudia}
}
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