Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells
Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells
Loading...
Date
2006
Authors
Langhorst, Matthias F.
Luxenhofer, Georg
Boneberg, Eva-Maria
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
The FASEB Journal ; 20 (2006), 6. - pp. 711-713. - ISSN 0892-6638. - eISSN 1530-6860
Abstract
T cell activation after contact with an antigen-presenting cell depends on the regulated assembly of the T cell receptor signaling complex, which involves the polarized assembly of a stable, raftlike macrodomain surrounding engaged T cell receptors. Here we show that the preformed reggie/flotillin caps present in resting T cells act as priming platforms for macrodomain assembly. Preformed reggie-1/flotillin-2 caps are exceptionally stable, as shown by fluorescence recovery after photobleaching (FRAP). Upon T cell stimulation, signaling molecules are recruited to the stable reggie/flotillin caps. Importantly, a trans-negative reggie-1/flotillin-2 deletion mutant, which interferes with assembly of the preformed reggie/flotillin cap, impairs raft polarization and macrodomain formation after T cell activation. Accordingly, expression of the trans-negative reggie-1 mutant leads to the incorrect positioning of the guanine nucleotide exchange factor Vav, resulting in defects in cytoskeletal reorganization. Thus, the preformed reggie/flotillin caps are stable priming platforms for the assembly of multiprotein complexes controlling actin reorganization during T cell activation.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
lipid rafts,signal transduction,T cell activation,actin cytoskeleton,raft clustering
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
LANGHORST, Matthias F., Alexander REUTER, Georg LUXENHOFER, Eva-Maria BONEBERG, Daniel LEGLER, Helmut PLATTNER, Claudia STÜRMER, 2006. Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells. In: The FASEB Journal. 20(6), pp. 711-713. ISSN 0892-6638. eISSN 1530-6860. Available under: doi: 10.1096/fj.05-4760fjeBibTex
@article{Langhorst2006-04Prefo-6738, year={2006}, doi={10.1096/fj.05-4760fje}, title={Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells}, number={6}, volume={20}, issn={0892-6638}, journal={The FASEB Journal}, pages={711--713}, author={Langhorst, Matthias F. and Reuter, Alexander and Luxenhofer, Georg and Boneberg, Eva-Maria and Legler, Daniel and Plattner, Helmut and Stürmer, Claudia} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/6738"> <dc:language>eng</dc:language> <dc:creator>Langhorst, Matthias F.</dc:creator> <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights> <dcterms:issued>2006-04</dcterms:issued> <dc:creator>Plattner, Helmut</dc:creator> <dc:format>application/pdf</dc:format> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:abstract xml:lang="eng">T cell activation after contact with an antigen-presenting cell depends on the regulated assembly of the T cell receptor signaling complex, which involves the polarized assembly of a stable, raftlike macrodomain surrounding engaged T cell receptors. Here we show that the preformed reggie/flotillin caps present in resting T cells act as priming platforms for macrodomain assembly. Preformed reggie-1/flotillin-2 caps are exceptionally stable, as shown by fluorescence recovery after photobleaching (FRAP). Upon T cell stimulation, signaling molecules are recruited to the stable reggie/flotillin caps. Importantly, a trans-negative reggie-1/flotillin-2 deletion mutant, which interferes with assembly of the preformed reggie/flotillin cap, impairs raft polarization and macrodomain formation after T cell activation. Accordingly, expression of the trans-negative reggie-1 mutant leads to the incorrect positioning of the guanine nucleotide exchange factor Vav, resulting in defects in cytoskeletal reorganization. Thus, the preformed reggie/flotillin caps are stable priming platforms for the assembly of multiprotein complexes controlling actin reorganization during T cell activation.</dcterms:abstract> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dcterms:title>Preformed reggie/flotillin caps : stable priming platforms for macrodomain assembly in T cells</dcterms:title> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:50Z</dcterms:available> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Boneberg, Eva-Maria</dc:contributor> <dc:creator>Legler, Daniel</dc:creator> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6738/1/113_Reggie_1_Flottilin_2_forms_stable_platforms.pdf"/> <dcterms:bibliographicCitation>First publ. in: The FASEB Journal ; 20 (2006), 6. - S. 711-713</dcterms:bibliographicCitation> <dc:contributor>Luxenhofer, Georg</dc:contributor> <dc:contributor>Stürmer, Claudia</dc:contributor> <dc:contributor>Legler, Daniel</dc:contributor> <dc:creator>Luxenhofer, Georg</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/6738/1/113_Reggie_1_Flottilin_2_forms_stable_platforms.pdf"/> <dc:contributor>Langhorst, Matthias F.</dc:contributor> <dc:creator>Stürmer, Claudia</dc:creator> <dc:creator>Boneberg, Eva-Maria</dc:creator> <dc:contributor>Reuter, Alexander</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:28:50Z</dc:date> <dc:creator>Reuter, Alexander</dc:creator> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/6738"/> <dc:contributor>Plattner, Helmut</dc:contributor> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes