Solution Structure of the N-terminal Zinc Fingers of the Xenopus laevis double-stranded RNA-binding Protein ZFa

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2005
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Martinez-Yamout, Maria A.
Dyson, H. Jane
Wright, Peter E.
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Several zinc finger proteins have been discovered recently that bind specifically to double-stranded RNA. These include the mammalian JAZ and wig proteins, and the seven-zinc finger protein ZFa from Xenopus laevis. We have determined the solution structure of a 127 residue fragment of ZFa, which consists of two zinc finger domains connected by a linker that remains unstructured in the free protein in solution. The first zinc finger consists of a three-stranded beta-sheet and three helices, while the second finger contains only a two-stranded sheet and two helices. The common structures of the core regions of the two fingers are superimposable. Each finger has a highly electropositive surface that maps to a helix-kink-helix motif. There is no evidence for interactions between the two fingers, consistent with the length (24 residues) and unstructured nature of the intervening linker. Comparison with a number of other proteins shows similarities in the topology and arrangement of secondary structure elements with canonical DNA-binding zinc fingers, with protein interaction motifs such as FOG zinc fingers, and with other DNA-binding and RNA-binding proteins that do not contain zinc. However, in none of these cases does the alignment of these structures with the ZFa zinc fingers produce a consistent picture of a plausible RNA-binding interface. We conclude that the ZFa zinc fingers represent a new motif for the binding of double-stranded RNA.

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ISO 690MÖLLER, Heiko M., Maria A. MARTINEZ-YAMOUT, H. Jane DYSON, Peter E. WRIGHT, 2005. Solution Structure of the N-terminal Zinc Fingers of the Xenopus laevis double-stranded RNA-binding Protein ZFa. In: Journal of Molecular Biology. 2005, 351(4), pp. 718-730. ISSN 0022-2836. eISSN 1089-8638. Available under: doi: 10.1016/j.jmb.2005.06.032
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@article{Moller2005-08-26Solut-21270,
  year={2005},
  doi={10.1016/j.jmb.2005.06.032},
  title={Solution Structure of the N-terminal Zinc Fingers of the Xenopus laevis double-stranded RNA-binding Protein ZFa},
  number={4},
  volume={351},
  issn={0022-2836},
  journal={Journal of Molecular Biology},
  pages={718--730},
  author={Möller, Heiko M. and Martinez-Yamout, Maria A. and Dyson, H. Jane and Wright, Peter E.}
}
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