A standalone editing protein deacylates mischarged canavanyl-tRNAArg to prevent canavanine incorporation into proteins

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Nucleic Acids Research. Oxford University Press. 2023, 51(5), pp. 2001-2010. ISSN 0305-1048. eISSN 1362-4962. Available under: doi: 10.1093/nar/gkac1197
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Error-free translation of the genetic code into proteins is vitally important for all organisms. Therefore, it is crucial that the correct amino acids are loaded onto their corresponding tRNAs. This process is highly challenging when aminoacyl-tRNA-synthetases encounter structural analogues to the native substrate like the arginine antimetabolite canavanine. To circumvent deleterious incorporation due to tRNA mischarging, editing mechanisms have evolved. However, only for half of the tRNA synthetases, editing activity is known and only few specific standalone editing proteins have been described. Understanding the diverse mechanisms resulting in error-free protein synthesis is of great importance. Here, we report the discovery of a protein that is upregulated upon canavanine stimulation in bacteria that live associated with canavanine-producing plants. We demonstrate that it acts as standalone editing protein specifically deacylating canavanylated tRNAArg. We therefore propose canavanyl-tRNAArgdeacylase (CtdA) as systematic name. Knockout strains show severe growth defects in canavanine-containing media and incorporate high amounts of canavanine into the proteome. CtdA is frequently found under control of guanidine riboswitches, revealing a functional connection of canavanine and guanidine metabolisms. Our results are the first to show editing activity towards mischarged tRNAArg and add to the puzzle of how faithful translation is ensured in nature.

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ISO 690HAUTH, Franziskus, Dietmar FUNCK, Jörg S. HARTIG, 2023. A standalone editing protein deacylates mischarged canavanyl-tRNAArg to prevent canavanine incorporation into proteins. In: Nucleic Acids Research. Oxford University Press. 2023, 51(5), pp. 2001-2010. ISSN 0305-1048. eISSN 1362-4962. Available under: doi: 10.1093/nar/gkac1197
BibTex
@article{Hauth2023stand-60055,
  year={2023},
  doi={10.1093/nar/gkac1197},
  title={A standalone editing protein deacylates mischarged canavanyl-tRNA<sup>Arg</sup> to prevent canavanine incorporation into proteins},
  number={5},
  volume={51},
  issn={0305-1048},
  journal={Nucleic Acids Research},
  pages={2001--2010},
  author={Hauth, Franziskus and Funck, Dietmar and Hartig, Jörg S.}
}
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