Structural transitions in short-chain lipid assemblies studied by 31 P-NMR spectroscopy

Lade...
Vorschaubild
Dateien
Zu diesem Dokument gibt es keine Dateien.
Datum
2002
Autor:innen
Tamm, Lukas K.
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Biophysical Journal. 2002, 83(2), pp. 994-1003. ISSN 0006-3495. eISSN 1542-0086
Zusammenfassung

The self-assembled supramolecular structures of diacylphosphatidylcholine (diCnPC), diacylphosphatidylethanolamine (diCnPE), diacylphosphatidyglycerol (diCnPG), and diacylphosphatidylserine (diCnPS) were investigated by 31P nuclear magnetic resonance (NMR) spectroscopy as a function of the hydrophobic acyl chain length. Short-chain homologs of these lipids formed micelles, and longer-chain homologs formed bilayers. The shortest acyl chain lengths that supported bilayer structures depended on the headgroup of the lipids. They increased in the order PE(C6)< PC(C9)≤ PS (C9 or C10)< PG(C11 or C12). This order correlated with the effective headgroup area, which is a function of the physical size, charge, hydration, and hydrogenbonding capacity of the four headgroups. Electrostatic screening of the headgroup charge with NaCl reduced the effective headgroup area of PS and PG and thereby decreased the micelle-to-bilayer transition of these lipid classes to shorter chain lengths. The experimentally determined supramolecular structures were compared to the assembly states predicted by packing constraints that were calculated from the hydrocarbon-chain volume and effective headgroup area of each lipid. The model accurately predicted the chain-length threshold for bilayer formation if the relative displacement of the acyl chains of the phospholipid were taken into account. The model also predicted cylindrical rather than spherical micelles for ll four diacylphospholipid classes and the 31P-NMR spectra provided evidence for a tubular network that appeared as an intermediate phase at the icelle-to-bilayer transition. The free energy of micellization per methylene group was independent of the structure of the supramolecular assembly, but was-0.95 kJ/mol (-0.23 kcal/mol) for the PGs compared to -2.5 kJ/mol (-0.60 kcal/mol) for the PCs. The integral membrane protein OmpA did not change the bilayer structure of thin (diC10PC) bilayers.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690KLEINSCHMIDT, Jörg, Lukas K. TAMM, 2002. Structural transitions in short-chain lipid assemblies studied by 31 P-NMR spectroscopy. In: Biophysical Journal. 2002, 83(2), pp. 994-1003. ISSN 0006-3495. eISSN 1542-0086
BibTex
@article{Kleinschmidt2002Struc-7264,
  year={2002},
  title={Structural transitions in short-chain lipid assemblies studied by 31 P-NMR spectroscopy},
  number={2},
  volume={83},
  issn={0006-3495},
  journal={Biophysical Journal},
  pages={994--1003},
  author={Kleinschmidt, Jörg and Tamm, Lukas K.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7264">
    <dcterms:bibliographicCitation>First publ. in: Biophysical Journal 83 (2002), 2, pp. 994-1003</dcterms:bibliographicCitation>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:format>application/pdf</dc:format>
    <dc:creator>Kleinschmidt, Jörg</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:04Z</dc:date>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7264"/>
    <dc:contributor>Kleinschmidt, Jörg</dc:contributor>
    <dcterms:title>Structural transitions in short-chain lipid assemblies studied by 31 P-NMR spectroscopy</dcterms:title>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:04Z</dcterms:available>
    <dc:contributor>Tamm, Lukas K.</dc:contributor>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7264/1/Structural_transitions_in_short_chain_lipid_assemblies_studied_by_31_P_NMR_spectroscopy.pdf"/>
    <dcterms:issued>2002</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7264/1/Structural_transitions_in_short_chain_lipid_assemblies_studied_by_31_P_NMR_spectroscopy.pdf"/>
    <dc:creator>Tamm, Lukas K.</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:abstract xml:lang="eng">The self-assembled supramolecular structures of diacylphosphatidylcholine (diCnPC), diacylphosphatidylethanolamine (diCnPE), diacylphosphatidyglycerol (diCnPG), and diacylphosphatidylserine (diCnPS) were investigated by 31P nuclear magnetic resonance (NMR) spectroscopy as a function of the hydrophobic acyl chain length. Short-chain homologs of these lipids formed micelles, and longer-chain homologs formed bilayers. The shortest acyl chain lengths that supported bilayer structures depended on the headgroup of the lipids. They increased in the order PE(C6)&lt; PC(C9)≤ PS (C9 or C10)&lt; PG(C11 or C12). This order correlated with the effective headgroup area, which is a function of the physical size, charge, hydration, and hydrogenbonding capacity of the four headgroups. Electrostatic screening of the headgroup charge with NaCl reduced the effective headgroup area of PS and PG and thereby decreased the micelle-to-bilayer transition of these lipid classes to shorter chain lengths. The experimentally determined supramolecular structures were compared to the assembly states predicted by packing constraints that were calculated from the hydrocarbon-chain volume and effective headgroup area of each lipid. The model accurately predicted the chain-length threshold for bilayer formation if the relative displacement of the acyl chains of the phospholipid were taken into account. The model also predicted cylindrical rather than spherical micelles for ll four diacylphospholipid classes and the 31P-NMR spectra provided evidence for a tubular network that appeared as an intermediate phase at the icelle-to-bilayer transition. The free energy of micellization per methylene group was independent of the structure of the supramolecular assembly, but was-0.95 kJ/mol (-0.23 kcal/mol) for the PGs compared to -2.5 kJ/mol (-0.60 kcal/mol) for the PCs. The integral membrane protein OmpA did not change the bilayer structure of thin (diC10PC) bilayers.</dcterms:abstract>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen