Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)

Welte, Wolfram; Leonhard, Monika; Diederichs, Kay; Weltzien, Hans-Ulrich; Restall, Colin; Hall, Christine; Chapman, Dennis

Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)

Files

Welte_208757.pdf(890.13 KB)

Date

1989

Authors

Welte, Wolfram

Leonhard, Monika

Diederichs, Kay

Weltzien, Hans-Ulrich

Restall, Colin

Hall, Christine

Chapman, Dennis

Publication type

Journal article

Published in

Biochimica et Biophysica Acta (BBA) - Biomembranes ; 984 (1989), 2. - pp. 193-199. - ISSN 0005-2736

Abstract

The (Ca2++Mg2+)-ATPase from sarcoplasmic reticulum (SR) has been solubilized with 1-alkanoyl propanediol 3-phosphorylcholines with chainlengths ranging between 8 and 12 C atoms. A marked dependence of the ATPase activity upon the chainlength was found, indicating that alkyl chainlengths with 12 C atoms are necessary for retention of activity. Addition of poly(ethylene glycol) to the eluting buffers used for gel filtration of the ATPase-detergent micelles was found to increase the activity and the long-term stability significantly. In the presence of Ca2+, the elution volume indicated an ATPase dimer, whereas in the absence of Ca2+ the elution volume indicated a monomeric solution. The purity of the preparations after gel filtration was improved by subsequent chromatography with a hydroxyapatite column.

Subject (DDC)

570 Biosciences, Biology

Keywords

ATPase,Ca2+-,enzyme stability,detergent,poly(ethylene glycol),(sarcoplasmic reticulum)

Cite This

ISO 690

WELTE, Wolfram, Monika LEONHARD, Kay DIEDERICHS, Hans-Ulrich WELTZIEN, Colin RESTALL, Christine HALL, Dennis CHAPMAN, 1989. Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol). In: Biochimica et Biophysica Acta (BBA) - Biomembranes. 984(2), pp. 193-199. ISSN 0005-2736. Available under: doi: 10.1016/0005-2736(89)90216-2

BibTex

@article{Welte1989Stabi-20875,
  year={1989},
  doi={10.1016/0005-2736(89)90216-2},
  title={Stabilization of detergent-solubilized Ca2+ -ATPase by poly(ethylene glycol)},
  number={2},
  volume={984},
  issn={0005-2736},
  journal={Biochimica et Biophysica Acta (BBA) - Biomembranes},
  pages={193--199},
  author={Welte, Wolfram and Leonhard, Monika and Diederichs, Kay and Weltzien, Hans-Ulrich and Restall, Colin and Hall, Christine and Chapman, Dennis}
}

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